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Hepatocyte Transplantation

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Peggy Stock

    1. Department of Surgery, University of Leipzig, Leipzig, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Bruno Christ

    1. Department of Surgery, University of Leipzig, Leipzig, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge method and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1506)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Hepatocyte Transplantation in Special Populations: Clinical Use in Children

      • Zahida Khan, Stephen C. Strom
      Pages 3-16

    3. Alternative Cell Sources to Adult Hepatocytes for Hepatic Cell Therapy

      • Eugenia Pareja, María José Gómez-Lechón, Laia Tolosa
      Pages 17-42

  3. Experimental Hepatocyte Transplantation

    1. Front Matter

      Pages 43-43
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    2. Late Gestation Fetal Hepatocytes for Liver Repopulation in the Rat

      • Jennifer A. Sanders
      Pages 45-60

    3. A Modified Protocol for the Isolation of Primary Human Hepatocytes with Improved Viability and Function from Normal and Diseased Human Liver

      • David C. Bartlett, Philip N. Newsome
      Pages 61-73

    4. Thy-1 (CD90)-Positive Hepatic Progenitor Cells, Hepatoctyes, and Non-parenchymal Liver Cells Isolated from Human Livers

      • Thomas S. Weiss, Rania Dayoub
      Pages 75-89

    5. Propagation of Human Hepatocytes in uPA/SCID Mice: Producing Chimeric Mice with Humanized Liver

      • Hiroki Ohshita, Chise Tateno
      Pages 91-100

    6. Fetal Liver Stem/Progenitor Cell Transplantation: A Model to Study Tissue Mass Replacement and Cell-Based Therapies

      • Mladen I. Yovchev, Michael Oertel
      Pages 101-115

    7. Successful Engraftment of Human Hepatocytes in uPA-SCID and FRG® KO Mice

      • Lander Foquet, Elizabeth M. Wilson, Lieven Verhoye, Markus Grompe, Geert Leroux-Roels, John Bial et al.
      Pages 117-130

    8. Gunn Rats as a Surrogate Model for Evaluation of Hepatocyte Transplantation-Based Therapies of Crigler–Najjar Syndrome Type 1

      • Zsuzsanna Polgar, Yanfeng Li, Xia Li Wang, Chandan Guha, Namita Roy-Chowdhury, Jayanta Roy-Chowdhury
      Pages 131-147

    9. Experimental Hepatocyte Transplantation in Pigs

      • Eiji Kobayashi, Shin Enosawa, Hiroshi Nagashima
      Pages 149-160

    10. Demonstrating Potential of Cell Therapy for Wilson’s Disease with the Long-Evans Cinnamon Rat Model

      • Fadi Luc Jaber, Yogeshwar Sharma, Sanjeev Gupta
      Pages 161-178

    11. Use of Thymidine Kinase Recombinant Adenovirus and Ganciclovir Mediated Mouse Liver Preconditioning for Hepatocyte Xenotransplantation

      • Daniel Moreno, Leire Neri, Eva Vicente, Africa Vales, Rafael Aldabe
      Pages 179-192

    12. Minimally Invasive Liver Preconditioning for Hepatocyte Transplantation in Rats

      • Martin Gaillard, Ibrahim Dagher
      Pages 193-200

    13. Preclinical Swine Models for Monitoring of Hepatocyte Transplantation by MRI

      • Nathanael Raschzok, Ulf Teichgräber, Johann Pratschke, Igor M. Sauer
      Pages 201-214

    14. Deep Digging: Far Red Imaging for the Monitoring of Transplanted Hepatocytes in Rats

      • Peggy Stock, Kristin Weber
      Pages 215-228

  4. Clinical Hepatocyte Transplantation

    1. Front Matter

      Pages 229-229
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    2. Isolation of GMP Grade Human Hepatocytes from Remnant Liver Tissue of Living Donor Liver Transplantation

      • Shin Enosawa
      Pages 231-245
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Keywords

About this book

This volume features up-to-date protocols for the isolation, preservation, and validation of various cell sources comprising large and small animal models, examining the impact of cell transplantation on acute and chronic liver diseases. Hepatocyte Transplantation: Methods and Protocols guides readers through laboratory protocols for the generation of humanized livers for the assessment of biological actions in vivo and techniques to monitor cell engraftment after cell transplantation in vivo are described and procedures for computational analyses of hepatocyte transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Hepatocyte Transplantation: Methods and Protocols is an essential resource for researchers and clinicians to assess the biological as well as the therapeutic potential of hepatocyte transplantation.


Editors and Affiliations

  • Department of Surgery, University of Leipzig, Leipzig, Germany

    Peggy Stock, Bruno Christ

Bibliographic Information

  • Book Title: Hepatocyte Transplantation

  • Book Subtitle: Methods and Protocols

  • Editors: Peggy Stock, Bruno Christ

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6506-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6504-5Published: 10 November 2016

  • Softcover ISBN: 978-1-4939-8221-9Published: 27 June 2018

  • eBook ISBN: 978-1-4939-6506-9Published: 09 November 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 365

  • Number of Illustrations: 16 b/w illustrations, 52 illustrations in colour

  • Topics: Cell Biology

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