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RNA Vaccines

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Thomas Kramps

    1. Boehringer Ingelheim Pharma GmbH & Co. KG, Ingelheim am Rhein, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Knut Elbers

    1. Boehringer Ingelheim GmbH, Ingelheim am Rhein, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1499)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Introduction to RNA Vaccines

    • Thomas Kramps, Knut Elbers
    Pages 1-11

  3. Self-Replicating RNA Vectors

    1. Front Matter

      Pages 13-13
      Download chapter PDF

    2. Self-Replicating RNA

      • Birke Andrea Tews, Gregor Meyers
      Pages 15-35

    3. Self-Replicating RNA Vaccine Delivery to Dendritic Cells

      • Thomas Démoulins, Pavlos C. Englezou, Panagiota Milona, Nicolas Ruggli, Nicola Tirelli, Chantal Pichon et al.
      Pages 37-75

    4. Plant Expression of Trans-Encapsidated Viral Nanoparticle Vaccines with Animal RNA Replicons

      • Yiyang Zhou, Alison A. McCormick, Christopher M. Kearney
      Pages 77-86

  4. Non-replicating mRNA Vectors

    1. Front Matter

      Pages 87-87
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    2. RNActive® Technology: Generation and Testing of Stable and Immunogenic mRNA Vaccines

      • Susanne Rauch, Johannes Lutz, Aleksandra Kowalczyk, Thomas Schlake, Regina Heidenreich
      Pages 89-107

    3. Nucleoside Modified mRNA Vaccines for Infectious Diseases

      • Norbert Pardi, Drew Weissman
      Pages 109-121

    4. Generation and Evaluation of Prophylactic mRNA Vaccines Against Allergy

      • Richard Weiss, Sandra Scheiblhofer, Josef Thalhamer
      Pages 123-139

  5. Adjuvantation and Delivery

    1. Front Matter

      Pages 141-141
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    2. Measuring the Adjuvant Activity of RNA Vaccines

      • Norbert Pardi, Drew Weissman
      Pages 143-153

    3. Generation of Immunostimulating 130 nm Protamine–RNA nanoparticles

      • Marina Tusup, Steve Pascolo
      Pages 155-163

    4. Electroporation of mRNA as Universal Technology Platform to Transfect a Variety of Primary Cells with Antigens and Functional Proteins

      • Kerstin F. Gerer, Stefanie Hoyer, Jan Dörrie, Niels Schaft
      Pages 165-178

    5. Adjuvant-Enhanced mRNA Vaccines

      • Lukasz Bialkowski, Kevin Van der Jeught, Dries Renmans, Alexia van Weijnen, Carlo Heirman, Marleen Keyaerts et al.
      Pages 179-191

    6. Enhanced Delivery of DNA or RNA Vaccines by Electroporation

      • Kate E. Broderick, Laurent M. Humeau
      Pages 193-200

  6. Preclinical and Clinical Development

    1. Front Matter

      Pages 201-201
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    2. The European Regulatory Environment of RNA-Based Vaccines

      • Thomas Hinz, Kajo Kallen, Cedrik M. Britten, Bruno Flamion, Ulrich Granzer, Axel Hoos et al.
      Pages 203-222

    3. Discovery and Subtyping of Neo-Epitope Specific T-Cell Responses for Cancer Immunotherapy: Addressing the Mutanome

      • Mustafa Diken, Mathias Vormehr, Christian Grunwitz, Sebastian Kreiter, Özlem Türeci, Ugur Sahin
      Pages 223-236

    4. Considerations for Producing mRNA Vaccines for Clinical Trials

      • Andreas Schmid
      Pages 237-251
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Keywords

About this book

This volume provides an overview of the field and practical hints for vaccinologists in academia and industry. Chapters provide protocols on self-replicating RNA vectors, non-replication mRNA vectors, adjuvantation and delivery, and preclinical and clinical development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, RNA Vaccines: Methods and Protocols aims to increased collaboration on RNA vaccines between basic and applied scientists in academia, government, and industry to develop future solutions for today’s challenges.


Editors and Affiliations

  • Boehringer Ingelheim Pharma GmbH & Co. KG, Ingelheim am Rhein, Germany

    Thomas Kramps

  • Boehringer Ingelheim GmbH, Ingelheim am Rhein, Germany

    Knut Elbers

Bibliographic Information

  • Book Title: RNA Vaccines

  • Book Subtitle: Methods and Protocols

  • Editors: Thomas Kramps, Knut Elbers

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6481-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6479-6Published: 17 December 2016

  • Softcover ISBN: 978-1-4939-8214-1Published: 07 July 2018

  • eBook ISBN: 978-1-4939-6481-9Published: 15 December 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 282

  • Number of Illustrations: 16 b/w illustrations, 26 illustrations in colour

  • Topics: Immunology

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