Bioreactors in Stem Cell Biology

1. Front Matter

   Pages i-xiv

   PDF

2. Multicompartmental Hollow-Fiber-Based Bioreactors for Dynamic Three-Dimensional Perfusion Culture

   • Eva Schmelzer, Jörg C. Gerlach

   Pages 1-19

3. A Bioreactor to Apply Multimodal Physical Stimuli to Cultured Cells

   • Jan-Christoph Edelmann, Lizzie Jones, Remi Peyronnet, Liang Lu, Peter Kohl, Ursula Ravens

   Pages 21-33

4. Aggregate and Microcarrier Cultures of Human Pluripotent Stem Cells in Stirred-Suspension Systems

   • Preeti Ashok, Yongjia Fan, Mahboubeh R. Rostami, Emmanuel S. Tzanakakis

   Pages 35-52

5. Expansion of Human Induced Pluripotent Stem Cells in Stirred Suspension Bioreactors

   • Walaa Almutawaa, Leili Rohani, Derrick E. Rancourt

   Pages 53-61

6. Uniform Embryoid Body Production and Enhanced Mesendoderm Differentiation with Murine Embryonic Stem Cells in a Rotary Suspension Bioreactor

   • Xiaohua Lei, Zhili Deng, Enkui Duan

   Pages 63-75

7. Expansion of Human Mesenchymal Stem Cells in a Microcarrier Bioreactor

   • Ang-Chen Tsai, Teng Ma

   Pages 77-86

8. Large-Scale Expansion and Differentiation of Mesenchymal Stem Cells in Microcarrier-Based Stirred Bioreactors

   • Sébastien Sart, Spiros N. Agathos

   Pages 87-102

9. Bioreactor Expansion of Skin-Derived Precursor Schwann Cells

   • Tylor Walsh, Jeff Biernaskie, Rajiv Midha, Michael S. Kallos

   Pages 103-110

10. Use of Stirred Suspension Bioreactors for Male Germ Cell Enrichment

    • Sadman Sakib, Camila Dores, Derrick Rancourt, Ina Dobrinski

    Pages 111-118

11. Generation of Neural Progenitor Spheres from Human Pluripotent Stem Cells in a Suspension Bioreactor

    • Yuanwei Yan, Liqing Song, Ang-Chen Tsai, Teng Ma, Yan Li

    Pages 119-128

12. Perfusion Stirred-Tank Bioreactors for 3D Differentiation of Human Neural Stem Cells

    • Daniel Simão, Francisca Arez, Ana P. Terasso, Catarina Pinto, Marcos F. Q. Sousa, Catarina Brito et al.

    Pages 129-142

13. Scalable Expansion of Human Pluripotent Stem Cell-Derived Neural Progenitors in Stirred Suspension Bioreactor Under Xeno-free Condition

    • Shiva Nemati, Saeed Abbasalizadeh, Hossein Baharvand

    Pages 143-158

14. A Microfluidic Bioreactor for Toxicity Testing of Stem Cell Derived 3D Cardiac Bodies

    • Jonas Christoffersson, Gunnar Bergström, Kristin Schwanke, Henning Kempf, Robert Zweigerdt, Carl-Fredrik Mandenius

    Pages 159-168

15. Novel Bioreactor Platform for Scalable Cardiomyogenic Differentiation from Pluripotent Stem Cell-Derived Embryoid Bodies

    • Sasitorn Rungarunlert, Joao N. Ferreira, Andras Dinnyes

    Pages 169-179

16. Whole-Heart Construct Cultivation Under 3D Mechanical Stimulation of the Left Ventricle

    • Jörn Hülsmann, Hug Aubin, Alexander Wehrmann, Alexander Jenke, Artur Lichtenberg, Payam Akhyari

    Pages 181-194

17. Tendon Differentiation on Decellularized Extracellular Matrix Under Cyclic Loading

    • Daniel W. Youngstrom, Jennifer G. Barrett

    Pages 195-202

18. Bioengineered Models of Solid Human Tumors for Cancer Research

    • Alessandro Marturano-Kruik, Aranzazu Villasante, Gordana Vunjak-Novakovic

    Pages 203-211

19. Development of a Bladder Bioreactor for Tissue Engineering in Urology

    • Niall F. Davis, Anthony Callanan

    Pages 213-221

20. Use of Microfluidic Technology to Monitor the Differentiation and Migration of Human ESC-Derived Neural Cells

    • Jiwoo Bae, Nayeon Lee, Wankyu Choi, Suji Lee, Jung Jae Ko, Baek Soo Han et al.

    Pages 223-235

This meticulous volume recognizes the need to translate what has been learned primarily in tissue culture dishes to approaches supporting scale-up studies, not only to large quantities of cells but also to heterogeneous cell constructs.  Notable advances are being made in these latter approaches, prompting this collection of a variety of representative protocols that facilitate important modifications and novel approaches to bioreactors in stem cell research, contributed by both established and new investigators in this area. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Practical and authoritative, Bioreactors in Stem Cell Biology: Methods and Protocols will serve as an ideal guide for scientists seeking to increase our understanding of stem cells and their potential to repair and regenerate tissues and organs.

• Biomaterials
• Stem cells
• Regenerative medicine
• Tissue and organ repair
• Heterogeneous cell constructs

• Ottawa Hospital Research Institute , Ottawa, Canada

  Kursad Turksen

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