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  • © 2017

Mammary Gland Development

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1501)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
  2. In Vivo Models

    1. Front Matter

      Pages 19-19
    2. RNA Profiling of Non-cultured Fibroblasts Isolated from Pubertal Mouse Mammary Gland Sections

      • Ayman M. Ibrahim, Claire Cairney, Joanna S. Morris, Torsten Stein
      Pages 149-164
    3. Analysis of the Involuting Mouse Mammary Gland: An In Vivo Model for Cell Death

      • Bethan Lloyd-Lewis, Timothy J. Sargeant, Peter A. Kreuzaler, Henrike K. Resemann, Sara Pensa, Christine J. Watson
      Pages 165-186
  3. In Vitro 2D & 3D-Models

    1. Front Matter

      Pages 187-187
    2. Contractility Assay for Established Myoepithelial Cell Lines

      • Stéphanie Cagnet, Marina A. Glukhova, Karine Raymond
      Pages 189-198
    3. Next Generation RNA Sequencing Analysis Reveals Expression of a Transient EMT Profile During Early Organization of MCF10A Cells in 3D

      • Benedikt Minke, Drieke Vandamme, Thomas Schwarzl, Walter Kolch, Finian Martin
      Pages 233-243
    4. A 3D Culture Model to Study How Fluid Pressure and Flow Affect the Behavior of Aggregates of Epithelial Cells

      • Alexandra S. Piotrowski-Daspit, Allison K. Simi, Mei-Fong Pang, Joe Tien, Celeste M. Nelson
      Pages 245-257
  4. Mammary Stem Cells

    1. Front Matter

      Pages 259-259
    2. Lineage Tracing of Mammary Stem and Progenitor Cells

      • Anoeska A. A. van de Moosdijk, Nai Yang Fu, Anne C. Rios, Jane E. Visvader, Renée van Amerongen
      Pages 291-308
  5. Translational Applications

    1. Front Matter

      Pages 309-309

About this book

This volume provides key molecular biological protocols and experimental strategies currently employed to study the various stages of mammary gland development. Chapters are divided into four parts covering genetically modified mouse models that exhibit prenatal and pubertal mammary gland phenotypes, 2D and 3D-model culture systems, methodologies for mammary stem cell isolation, and translational applications that provide a bridge between experimental studies of mammary gland development and the study of human breast cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mammary Gland Development: Methods and Protocols  will be useful for researchers whose primary interest is in mammary gland development; developmental biologists, epithelial cell biologists,  and those with an interest in molecular mechanisms underlying breast cancer.

Editors and Affiliations

  • School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Ireland

    Finian Martin

  • Institute of Cancer Sciences College of MVLS University of Glasgow, Glasgow, United Kingdom

    Torsten Stein

  • Division of Oncology-Pathology, Lund University Cancer Center/Medicon Village, Lund, Sweden

    Jillian Howlin

Bibliographic Information

  • Book Title: Mammary Gland Development

  • Book Subtitle: Methods and Protocols

  • Editors: Finian Martin, Torsten Stein, Jillian Howlin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6475-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6473-4Published: 29 October 2016

  • Softcover ISBN: 978-1-4939-8212-7Published: 10 July 2018

  • eBook ISBN: 978-1-4939-6475-8Published: 28 October 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 358

  • Number of Illustrations: 30 b/w illustrations, 32 illustrations in colour

  • Topics: Molecular Medicine

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access