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In Vitro Mutagenesis

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Andrew Reeves

    1. Coskata, Inc., Warrenville, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1498)

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Table of contents (34 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Gene and Genome-Editing Methods Part I

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells

      • Ciaran M. Lee, Haibao Zhu, Timothy H. Davis, Harshahardhan Deshmukh, Gang Bao
      Pages 3-21

    3. Mutagenesis and Genome Engineering of Epstein–Barr Virus in Cultured Human Cells by CRISPR/Cas9

      • Kit-San Yuen, Chi-Ping Chan, Kin-Hang Kok, Dong-Yan Jin
      Pages 23-31

    4. Use of CRISPR/Cas Genome Editing Technology for Targeted Mutagenesis in Rice

      • Rongfang Xu, Pengcheng Wei, Jianbo Yang
      Pages 33-40

    5. All-in-One CRISPR-Cas9/FokI-dCas9 Vector-Mediated Multiplex Genome Engineering in Cultured Cells

      • Tetsushi Sakuma, Takuya Sakamoto, Takashi Yamamoto
      Pages 41-56

    6. CRISPR/Cas9-Mediated Mutagenesis of Human Pluripotent Stem Cells in Defined Xeno-Free E8 Medium

      • Chew-Li Soh, Danwei Huangfu
      Pages 57-78

    7. Development of CRISPR/Cas9 for Efficient Genome Editing in Toxoplasma gondii

      • Bang Shen, Kevin Brown, Shaojun Long, L. David Sibley
      Pages 79-103

  4. Bioinformatics Approaches for Identifying and Analyzing Mutagenesis Targets

    1. Front Matter

      Pages 179-179
      Download chapter PDF

    2. In Silico Approaches to Identify Mutagenesis Targets to Probe and Alter Protein–Cofactor and Protein–Protein Functional Relationships

      • Brian A. Dow, Esha Sehanobish, Victor L. Davidson
      Pages 181-190

    3. In Silico Prediction of Deleteriousness for Nonsynonymous and Splice-Altering Single Nucleotide Variants in the Human Genome

      • Xueqiu Jian, Xiaoming Liu
      Pages 191-197

    4. In Silico Methods for Analyzing Mutagenesis Targets

      • Troy C. Messina
      Pages 199-226

    5. Methods for Detecting Critical Residues in Proteins

      • Nurit Haspel, Filip Jagodzinski
      Pages 227-242

    6. A Method for Bioinformatic Analysis of Transposon Insertion Sequencing (INSeq) Results for Identification of Microbial Fitness Determinants

      • Nengding Wang, Egon A. Ozer
      Pages 243-253
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Keywords

About this book

In vitro mutagenesis remains a critical experimental approach for investigating gene and protein function at the cellular level. This volume provides a wide variety of updated and novel approaches for performing in vitro mutagenesis using such methods as genome editing, transposon (Tn) mutagenesis, site-directed, and random mutagenesis. In Vitro Mutagenesis: Methods and Protocols guides readers through methods for gene and genome editing, practical bioinformatics approaches for identifying mutagenesis targets, and novel site-directed and random mutagenesis approaches aimed at gaining a better understanding of protein-protein and protein-cofactor interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.

 

Authoritative and cutting-edge, In Vitro Mutagenesis: Methods and Protocols aims to provide a highly accessible and practical manual for current and future molecular biology researchers, from the beginner practitioner to the advanced investigator in fields such as molecular genetics, biochemistry, and biochemical and metabolic engineering.

Editors and Affiliations

  • Coskata, Inc., Warrenville, USA

    Andrew Reeves

Bibliographic Information

  • Book Title: In Vitro Mutagenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Andrew Reeves

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6472-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6470-3Published: 06 October 2016

  • Softcover ISBN: 978-1-4939-8211-0Published: 27 June 2018

  • eBook ISBN: 978-1-4939-6472-7Published: 05 October 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 511

  • Number of Illustrations: 44 b/w illustrations, 52 illustrations in colour

  • Topics: Human Genetics

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