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The Golgi Complex

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. William J. Brown

    1. Dept of Molecular Biology & Genetics, Cornell University, Ithaca, USA

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  • Includes cutting-edge methods and protocols for Golgi complex research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1496)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. 4D Confocal Imaging of Yeast Organelles

    • Kasey J. Day, Effrosyni Papanikou, Benjamin S. Glick
    Pages 1-11

  3. Imaging the Polarized Sorting of Proteins from the Golgi Complex in Live Neurons

    • Ginny G. Farías, Dylan J. Britt, Juan S. Bonifacino
    Pages 13-30

  4. Imaging Golgi Outposts in Fixed and Living Neurons

    • Mariano Bisbal, Gonzalo Quassollo, Alfredo Caceres
    Pages 31-39

  5. Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor

    • Jon E. Paczkowski, J. Christopher Fromme
    Pages 41-53

  6. STEM Tomography Imaging of Hypertrophied Golgi Stacks in Mucilage-Secreting Cells

    • Byung-Ho Kang
    Pages 55-62

  7. Reconstitution of COPI Vesicle and Tubule Formation

    • Seung-Yeol Park, Jia-Shu Yang, Victor W. Hsu
    Pages 63-74

  8. Reconstitution of Phospholipase A2-Dependent Golgi Membrane Tubules

    • Edward B. Cluett, Paul de Figueiredo, Marie E. Bechler, Kevin D. Thorsen, William J. Brown
    Pages 75-90

  9. Proteomic Characterization of Golgi Membranes Enriched from Arabidopsis Suspension Cell Cultures

    • Sara Fasmer Hansen, Berit Ebert, Carsten Rautengarten, Joshua L. Heazlewood
    Pages 91-109

  10. High-Content Analysis of the Golgi Complex by Correlative Screening Microscopy

    • Manuel Gunkel, Holger Erfle, Vytaute Starkuviene
    Pages 111-121

  11. Activity Detection of GalNAc Transferases by Protein-Based Fluorescence Sensors In Vivo

    • Lina Song, Collin Bachert, Adam D. Linstedt
    Pages 123-131

  12. In Situ Proximity Ligation Assay (PLA) Analysis of Protein Complexes Formed Between Golgi-Resident, Glycosylation-Related Transporters and Transferases in Adherent Mammalian Cell Cultures

    • Dorota Maszczak-Seneczko, Paulina Sosicka, Teresa Olczak, Mariusz Olczak
    Pages 133-143

  13. Creating Knockouts of Conserved Oligomeric Golgi Complex Subunits Using CRISPR-Mediated Gene Editing Paired with a Selection Strategy Based on Glycosylation Defects Associated with Impaired COG Complex Function

    • Jessica Bailey Blackburn, Vladimir V. Lupashin
    Pages 145-161

  14. Reversible Controlled Aggregation of Golgi Resident Enzymes to Assess Their Transport/Dynamics Along the Secretory Pathway

    • Riccardo Rizzo, Alberto Luini
    Pages 163-172

  15. Assays to Study the Fragmentation of the Golgi Complex During the G2–M Transition of the Cell Cycle

    • Inmaculada Ayala, Antonino Colanzi
    Pages 173-185

  16. The Role of Lysophospholipid Acyltransferases in the Golgi Complex

    • John A. Schmidt
    Pages 187-195

  17. Methods to Purify and Assay Secretory Pathway Kinases

    • Vincent S. Tagliabracci, Jianzhong Wen, Junyu Xiao
    Pages 197-215

  18. Back Matter

    Pages 217-219
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Keywords

About this book

Focusing on new methods and techniques developed to address longstanding questions about the Golgi complex, this volume explores a diverse set of chapters, ranging from live and fixed cell imaging techniques to in vitro biochemical reconstitution systems. Each chapter provides a detailed set of specific instructions, which should enable anyone to successfully complete the assays. Written for the highly successful Methods in Molecular Biology series program, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips from the experts on troubleshooting and avoiding known pitfalls. 

Practical and detailed, The Golgi Complex: Methods and Protocols will aid both new and established researchers in the field by providing strong hands-on instructions that can be directly applied to their research programs.

Editors and Affiliations

  • Dept of Molecular Biology & Genetics, Cornell University, Ithaca, USA

    William J. Brown

Bibliographic Information

  • Book Title: The Golgi Complex

  • Book Subtitle: Methods and Protocols

  • Editors: William J. Brown

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6463-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-6461-1Published: 23 September 2016

  • Softcover ISBN: 978-1-4939-8208-0Published: 04 May 2018

  • eBook ISBN: 978-1-4939-6463-5Published: 08 September 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 219

  • Number of Illustrations: 11 b/w illustrations, 33 illustrations in colour

  • Topics: Cell Biology

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