Skip to main content
  • Book
  • © 2017

Semaphorin Signaling

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1493)

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (34 protocols)

  1. Front Matter

    Pages i-xv
  2. Semaphorins and their Signaling Mechanisms

    • Laura Taylor Alto, Jonathan R. Terman
    Pages 1-25
  3. Biochemical Approaches

    1. Front Matter

      Pages 27-27
    2. Isolating Fc-Tagged SEMA4D Recombinant Protein from 293FT Cells

      • Joanne Soong, Glynis A. Scott
      Pages 29-40
    3. Plate-Based Assay for Measuring Direct Semaphorin–Neuropilin Interactions

      • Matthew W. Parker, Craig W. Vander Kooi
      Pages 73-87
    4. Characterizing Plexin GTPase Interactions Using Gel Filtration, Surface Plasmon Resonance Spectrometry, and Isothermal Titration Calorimetry

      • Jeannine Muller-Greven, SoonJeung Kim, Prasanta K. Hota, Yufeng Tong, Susmita Borthakur, Matthias Buck
      Pages 89-105
    5. Characterizing F-actin Disassembly Induced by the Semaphorin-Signaling Component MICAL

      • Jimok Yoon, Ruei-Jiun Hung, Jonathan R. Terman
      Pages 119-128
  4. In Vitro Approaches

    1. Front Matter

      Pages 161-161
    2. Using Heterologous COS-7 Cells to Identify Semaphorin-Signaling Components

      • Atsuko Sakurai, Colleen L. Doçi, J. Silvio Gutkind
      Pages 163-170
    3. Analysis of Semaphorin-Induced Growth Cone Collapse and Axon Growth Inhibition

      • Lionel A. T. Meyer, Andrius Kaselis, Saulius Satkauskas, Dominique Bagnard
      Pages 171-183
    4. Characterizing Semaphorin Signaling Using Isolated Neurons in Culture

      • Sophie Chauvet, Erik Mire, Fanny Mann
      Pages 223-235
    5. Performing Axon Orientation Assays with Secreted Semaphorins and Other Guidance Cues

      • Céline Delloye-Bourgeois, Frédéric Moret, Valérie Castellani
      Pages 237-246

About this book

This volume arranged into three sections describes biochemical, in vitro, and in vivo protocols on Semaphorins. Chapters focus on approaches that would allow the novice to study Semaphorins and employ robust assays to characterize mechanisms of action. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Semaphorin Signaling: Methods and Protocols aims to ensure successful results in the further study of this vital field.



Editors and Affiliations

  • Departments of Neuroscience and Pharmacology, The University of Texas Southwestern Medical Center, Dallas, USA

    Jonathan R. Terman

Bibliographic Information

  • Book Title: Semaphorin Signaling

  • Book Subtitle: Methods and Protocols

  • Editors: Jonathan R. Terman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6448-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6446-8Published: 28 October 2016

  • Softcover ISBN: 978-1-4939-8203-5Published: 14 July 2018

  • eBook ISBN: 978-1-4939-6448-2Published: 27 October 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 503

  • Number of Illustrations: 42 b/w illustrations, 54 illustrations in colour

  • Topics: Protein Science

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access