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Vaccine Adjuvants

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Christopher B. Fox

    1. Infectious Disease Research Institute, Seattle, USA

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  • Includes cutting-edge methods and protocols in the field of vaccine adjuvant research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1494)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Overview of Vaccine Adjuvants: Introduction, History, and Current Status

    • Ruchi R. Shah, Kimberly J. Hassett, Luis A. Brito
    Pages 1-13

  3. Development of the CpG Adjuvant 1018: A Case Study

    • John D. Campbell
    Pages 15-27

  4. Syntheses of Human TLR8-Specific Small-Molecule Agonists

    • Mallesh Beesu, Hari Prasad Kokatla, Sunil A. David
    Pages 29-44

  5. Semisynthesis of Analogues of the Saponin Immunoadjuvant QS-21

    • Alberto Fernández-Tejada, William E. Walkowicz, Derek S. Tan, David Y. Gin
    Pages 45-71

  6. QS-21 Adjuvant: Laboratory-Scale Purification Method and Formulation Into Liposomes

    • Livia Brunner, Christophe Barnier-Quer, Nicolas Collin
    Pages 73-86

  7. Purification of an Immunoadjuvant Saponin Fraction from Quillaja brasiliensis Leaves by Reversed-Phase Silica Gel Chromatography

    • Anna C. A. Yendo, Fernanda de Costa, Carla Kauffmann, Juliane D. Fleck, Grace Gosmann, Arthur G. Fett-Neto
    Pages 87-93

  8. Biosynthetic Approaches to Squalene Production: The Case of Yeast

    • Martin Valachovič, Ivan Hapala
    Pages 95-106

  9. In Silico Adjuvant Design and Validation

    • Matthew N. Davies, Helene Pere, Iris Bosschem, Freddy Haesebrouck, Bram Flahou, Eric Tartour et al.
    Pages 107-125

  10. Manufacturing Methods for Liposome Adjuvants

    • Yvonne Perrie, Elisabeth Kastner, Swapnil Khadke, Carla B. Roces, Peter Stone
    Pages 127-144

  11. Synthesis of Lymph Node-Targeting Adjuvants

    • Melissa C. Hanson, Darrell J. Irvine
    Pages 145-152

  12. Preparing an Adjuvanted Thermoresponsive Gel Formulation for Sublingual Vaccination

    • Manjari Lal, Jessica White, Changcheng Zhu
    Pages 153-163

  13. Manufacture of Oil-in-Water Emulsion Adjuvants

    • Jean Haensler
    Pages 165-180

  14. Methods to Prepare Aluminum Salt-Adjuvanted Vaccines

    • Sachin G. Thakkar, Zhengrong Cui
    Pages 181-199

  15. Production of Adjuvant-Loaded Biodegradable Particles for Use in Cancer Vaccines

    • Cristina Maria de Barros, Emad Ibrahim Wafa, Khanidtha Chitphet, Kawther Ahmed, Sean M. Geary, Aliasger K. Salem
    Pages 201-213

  16. Lyophilization of Adjuvanted Vaccines: Methods for Formulation of a Thermostable Freeze-Dried Product

    • Michelle Y. Chan, Timothy S. Dutill, Ryan M. Kramer
    Pages 215-226

  17. Stressed Stability Techniques for Adjuvant Formulations

    • Manvi Hasija, Anthony Sheung, Nausheen Rahman, Salvador F. Ausar
    Pages 227-238

  18. Particle Sizing of Nanoparticle Adjuvant Formulations by Dynamic Light Scattering (DLS) and Nanoparticle Tracking Analysis (NTA)

    • Michelle Y. Chan, Quinton M. Dowling, Sandra J. Sivananthan, Ryan M. Kramer
    Pages 239-252

  19. Quantification of Multiple Components of Complex Aluminum-Based Adjuvant Mixtures by Using Fourier Transform Infrared Spectroscopy and Partial Least Squares Modeling

    • Quinton M. Dowling, Ryan M. Kramer
    Pages 253-261

  20. Determination of Protein Content in Alhydrogel®-Based Vaccines by O-Phthalaldehyde Assay

    • Kelly M. Rausch, Daming Zhu
    Pages 263-271
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Keywords

About this book

This collection seeks to elucidate the practical methods necessary for successful adjuvant development, with a particular focus on the synthesis, formulation, manufacturing, and characterization aspects involved. Beginning with an overview and a case study, the book then delves into in silico design, chemical synthesis, biosynthesis, and/or purification from natural raw materials of specific adjuvant molecules, adjuvant formulation approaches, the analytical characterization of adjuvant formulations and adjuvant-containing vaccines, as well as the biological characterization of vaccine adjuvant activity, including in vitro and in vivo approaches, to measure innate and adaptive immune responses. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Vaccine Adjuvants: Methods and Protocols aims to facilitate vaccine adjuvant product development efforts, making them more accessible, manufacturable, and better characterized.

Editors and Affiliations

  • Infectious Disease Research Institute, Seattle, USA

    Christopher B. Fox

Bibliographic Information

  • Book Title: Vaccine Adjuvants

  • Book Subtitle: Methods and Protocols

  • Editors: Christopher B. Fox

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6445-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6443-7Published: 08 October 2016

  • Softcover ISBN: 978-1-4939-8202-8Published: 14 July 2018

  • eBook ISBN: 978-1-4939-6445-1Published: 07 October 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 372

  • Number of Illustrations: 42 b/w illustrations, 33 illustrations in colour

  • Topics: Immunology

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