Skip to main content
SpringerLink
Log in

Activity-Based Proteomics

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Herman S. Overkleeft

    1. Bio-Organic Synthesis Group Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Bogdan I. Florea

    1. Bio-Organic Synthesis Group Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1491)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Activity-Based Protein Profiling: From Chemical Novelty to Biomedical Stalwart

    • Sander I. van Kasteren, Bogdan I. Florea, Herman S. Overkleeft
    Pages 1-8

  3. Explorative Activity-Based Proteomics

    1. Front Matter

      Pages 9-9
      Download chapter PDF

    2. A Quantitative Mass-Spectrometry Platform to Monitor Changes in Cysteine Reactivity

      • Yu Qian, Eranthie Weerapana
      Pages 11-22

    3. Activity-Based Protein Profiling with Natural Product-Derived Chemical Probes in Human Cell Lysates

      • Susanne Zweerink, Tanja Pollmann, Sabrina Ninck, Farnusch Kaschani, Markus Kaiser
      Pages 23-46

    4. Inhibitor Discovery by Convolution ABPP

      • Balakumaran Chandrasekar, Tram Ngoc Hong, Renier A. L. van der Hoorn
      Pages 47-56

    5. Activity-Based Protein Profiling in Bacteria

      • Joanna Krysiak, Stephan A. Sieber
      Pages 57-74

    6. Activity-Based Lipid Esterase Profiling of M. bovis BCG at Different Metabolic States Using Tetrahydrolipstatin (THL) as Bait

      • Madhu Sudhan Ravindran, Markus R. Wenk
      Pages 75-85

    7. Comparative Activity-Based Flavin-Dependent Oxidase Profiling

      • Joanna Krysiak, Rolf Breinbauer
      Pages 87-99

  4. Medical Applications of Activity-Based Proteomics

    1. Front Matter

      Pages 101-101
      Download chapter PDF

    2. Activity-Dependent Photoaffinity Labeling of Metalloproteases

      • Laurette Prely, Theo Klein, Paul P. Geurink, Krisztina Paal, Herman S. Overkleeft, Rainer Bischoff
      Pages 103-111

    3. Profiling the Activity of Deubiquitinating Enzymes Using Chemically Synthesized Ubiquitin-Based Probes

      • Yves Leestemaker, Annemieke de Jong, Huib Ovaa
      Pages 113-130

    4. Biochemical and Mass Spectrometry-Based Approaches to Profile SUMOylation in Human Cells

      • Benedikt M. Kessler, Sara Bursomanno, Joanna F. McGouran, Ian D. Hickson, Ying Liu
      Pages 131-144

    5. Live Cell Imaging and Profiling of Cysteine Cathepsin Activity Using a Quenched Activity-Based Probe

      • Laura E. Edgington-Mitchell, Matthew Bogyo, Martijn Verdoes
      Pages 145-159

    6. Competitive ABPP of Serine Hydrolases: A Case Study on DAGL-Alpha

      • Marc P. Baggelaar, Mario Van der Stelt
      Pages 161-169

  5. Chemical Strategies in Activity-Based Proteomics

    1. Front Matter

      Pages 171-171
      Download chapter PDF

    2. A Protocol for Protein Profiling Using Chemoselective Cleavable Linker Probes in Semi-permeabilized Cells

      • Jasper H. L. Claessen, Martin D. Witte
      Pages 173-184

    3. Cleavable Linkers in Chemical Proteomics Applications

      • Yinliang Yang, Marko Fonović, Steven H. L. Verhelst
      Pages 185-203

    4. Two-Step Activity-Based Protein Profiling with the Proteasome System as Model of Study

      • Guillem Paniagua Soriano, Herman S. Overkleeft, Bogdan I. Florea
      Pages 205-215

  6. Back Matter

    Pages 217-222
    Download chapter PDF
Back to top

Keywords

About this book

This volume focuses on explorative activity-based proteomics, biomedical applications of activity-based proteomics, and chemical strategies in activity-based proteomics providing a concise overview of activity-based protein profiling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Activity-Based Proteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.


Editors and Affiliations

  • Bio-Organic Synthesis Group Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands

    Herman S. Overkleeft, Bogdan I. Florea

Bibliographic Information

  • Book Title: Activity-Based Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Herman S. Overkleeft, Bogdan I. Florea

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6439-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6437-6Published: 25 October 2016

  • Softcover ISBN: 978-1-4939-8200-4Published: 10 July 2018

  • eBook ISBN: 978-1-4939-6439-0Published: 24 October 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 222

  • Number of Illustrations: 29 b/w illustrations, 18 illustrations in colour

  • Topics: Protein Science

Publish with us

Policies and ethics

Back to top

Search

Navigation