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Optical Tweezers

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Arne Gennerich

    1. Department of Anatomy and Structural Biology & Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1486)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Theory and Design of Optical Tweezers

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Introduction to Optical Tweezers

      • Matthias D. Koch, Joshua W. Shaevitz
      Pages 3-24

    3. Exact Theory of Optical Tweezers and Its Application to Absolute Calibration

      • Rafael S. Dutra, Nathan B. Viana, Paulo A. Maia Neto, H. Moysés Nussenzveig
      Pages 25-39

    4. Beyond the Hookean Spring Model: Direct Measurement of Optical Forces Through Light Momentum Changes

      • Arnau Farré, Ferran Marsà, Mario Montes-Usategui
      Pages 41-76

    5. A Surface-Coupled Optical Trap with 1-bp Precision via Active Stabilization

      • Stephen R. Okoniewski, Ashley R. Carter, Thomas T. Perkins
      Pages 77-107

    6. Implementation and Tuning of an Optical Tweezers Force-Clamp Feedback System

      • Michael Bugiel, Anita Jannasch, Erik Schäffer
      Pages 109-136

    7. Custom-Made Microspheres for Optical Tweezers

      • Anita Jannasch, Mohammad K. Abdosamadi, Avin Ramaiya, Suman De, Valentina Ferro, Aaron Sonnberger et al.
      Pages 137-155

    8. Optical Torque Wrench Design and Calibration

      • Zhanna Santybayeva, Francesco Pedaci
      Pages 157-181

    9. High-Resolution “Fleezers”: Dual-Trap Optical Tweezers Combined with Single-Molecule Fluorescence Detection

      • Kevin D. Whitley, Matthew J. Comstock, Yann R. Chemla
      Pages 183-256

    10. Versatile Quadruple-Trap Optical Tweezers for Dual DNA Experiments

      • Iddo Heller, Niels Laurens, Daan Vorselen, Onno D. Broekmans, Andreas S. Biebricher, Graeme A. King et al.
      Pages 257-272

  3. Optical Tweezers-Based Manipulation and Analysis of DNA-DNA Interactions, Protein Unfolding/Refolding, and Motion/Force-Generation by Mechanoenzymes In Vitro

    1. Front Matter

      Pages 273-273
      Download chapter PDF

    2. Probing DNA–DNA Interactions with a Combination of Quadruple-Trap Optical Tweezers and Microfluidics

      • Ineke Brouwer, Graeme A. King, Iddo Heller, Andreas S. Biebricher, Erwin J. G. Peterman, Gijs J. L. Wuite
      Pages 275-293

    3. Probing Single Helicase Dynamics on Long Nucleic Acids Through Fluorescence-Force Measurement

      • Chang-Ting Lin, Taekjip Ha
      Pages 295-316

    4. Mechanically Watching the ClpXP Proteolytic Machinery

      • Juan Carlos Cordova, Adrian O. Olivares, Matthew J. Lang
      Pages 317-341

    5. Deciphering the Molecular Mechanism of the Bacteriophage φ29 DNA Packaging Motor

      • Shixin Liu, Sara Tafoya, Carlos Bustamante
      Pages 343-355

    6. Single-Molecule Protein Folding Experiments Using High-Precision Optical Tweezers

      • Junyi Jiao, Aleksander A. Rebane, Lu Ma, Yongli Zhang
      Pages 357-390

    7. Observing Single RNA Polymerase Molecules Down to Base-Pair Resolution

      • Anirban Chakraborty, Cong A. Meng, Steven M. Block
      Pages 391-409

    8. Optical Tweezers-Based Measurements of Forces and Dynamics at Microtubule Ends

      • Marian Baclayon, Svenja-Marei Kalisch, Ed Hendel, Liedewij Laan, Julien Husson, E. Laura Munteanu et al.
      Pages 411-435

    9. Simultaneous Manipulation and Super-Resolution Fluorescence Imaging of Individual Kinetochores Coupled to Microtubule Tips

      • Yi Deng, Charles L. Asbury
      Pages 437-467
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Keywords

About this book

The aim of this volume is to provide a comprehensive overview of optical tweezers setups, both in practical and theoretical terms, to help biophysicists, biochemists, and cell biologists to build and calibrate their own instruments and to perform force measurements on mechanoenzymes both in isolation in vitro and in living cells. Chapters have been divided in three parts focusing on theory and practical design of optical tweezers, detailed protocols for performing force measurements on single DNA- and microtubule/actin-associated mechanoenzymes in isolation, and describing recent advances that have opened up quantitative force measurements in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Optical Tweezers: Methods and Protocols aims help to further expand the accessibility and use of optical traps by scientists of diverse disciplines. 

Editors and Affiliations

  • Department of Anatomy and Structural Biology & Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, USA

    Arne Gennerich

Bibliographic Information

  • Book Title: Optical Tweezers

  • Book Subtitle: Methods and Protocols

  • Editors: Arne Gennerich

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6421-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6419-2Published: 15 November 2016

  • Softcover ISBN: 978-1-4939-8194-6Published: 23 June 2018

  • eBook ISBN: 978-1-4939-6421-5Published: 14 November 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 555

  • Number of Illustrations: 21 b/w illustrations, 133 illustrations in colour

  • Topics: Biotechnology

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