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  • © 2016

Polycomb Group Proteins

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1480)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xv
  2. Part I

    1. Front Matter

      Pages 1-1
    2. Chromatin Immunoprecipitation

      • Laura Wiehle, Achim Breiling
      Pages 7-21
    3. Chromatin Preparation and Chromatin Immuno-precipitation from Drosophila Embryos

      • Eva Löser, Daniel Latreille, Nicola Iovino
      Pages 23-36
    4. ChIP-seq Data Processing for PcG Proteins and Associated Histone Modifications

      • Ozren Bogdanovic´, Simon J. van Heeringen
      Pages 37-53
  3. Part II

    1. Front Matter

      Pages 67-67
    2. Noncoding RNA Interplay with the Genome

      • Davide Gabellini
      Pages 69-72
    3. RIP: RNA Immunoprecipitation

      • Miriam Gagliardi, Maria R. Matarazzo
      Pages 73-86
    4. Capture Hybridization Analysis of DNA Targets

      • Alec N. Sexton, Martin Machyna, Matthew D. Simon
      Pages 87-97
    5. Analysis RNA-seq and Noncoding RNA

      • Alberto Arrigoni, Valeria Ranzani, Grazisa Rossetti, Ilaria Panzeri, Sergio Abrignani, Raoul J. P. Bonnal et al.
      Pages 125-135

About this book

This volume presents the most recent technologies used in the Polycomb Group Proteins (PcG) field. Chapters detail state-of-the-art methods, creating a unique and comprehensive reference source for investigating Polycomb function in the nucleus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Polycomb Group Proteins: Method and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • IBCN-CNR, Institute of Cell Biology and Neurobiology and Istituto Nazionale di Genetica Molecolare “Romeo ed Enrica Invernizzi”, Rome, Italy

    Chiara Lanzuolo

  • Istituto Nazionale di Genetica Molecolare “Romeo ed Enrica Invernizzi”, Milan, Italy

    Beatrice Bodega

Bibliographic Information

  • Book Title: Polycomb Group Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Chiara Lanzuolo, Beatrice Bodega

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6380-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-6378-2Published: 23 September 2016

  • Softcover ISBN: 978-1-4939-8181-6Published: 27 June 2018

  • eBook ISBN: 978-1-4939-6380-5Published: 22 September 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 325

  • Number of Illustrations: 17 b/w illustrations, 23 illustrations in colour

  • Topics: Protein Science

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access