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Cell Microencapsulation

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Emmanuel C. Opara

    1. Wake Forest Institute for Regenerative Medicine Wake Forest School of Medicine, Winston-Salem, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains keynotes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1479)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Historical Perspectives and Current Challenges in Cell Microencapsulation

      • Paul de Vos
      Pages 3-21

    3. Applications of Cell Microencapsulation

      • Emmanuel C. Opara
      Pages 23-39

  3. Approaches to Cell Microencapsulation

    1. Front Matter

      Pages 41-41
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    2. Cell Microencapsulation: Dripping Methods

      • A. Bidoret, E. Martins, B. Poncelet De Smet, D. Poncelet
      Pages 43-55

    3. Field Effect Microparticle Generation for Cell Microencapsulation

      • Brend Ray-Sea Hsu, Shin-Huei Fu
      Pages 57-70

    4. Microfluidic Approach to Cell Microencapsulation

      • Varna Sharma, Michael Hunckler, Melur K. Ramasubramanian, Emmanuel C. Opara, Kalyan C. Katuri
      Pages 71-76

  4. Biomaterials and Enabling Technologies in Cell Microencapsulation

    1. Front Matter

      Pages 77-77
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    2. Polymeric Materials for Cell Microencapsulation

      • A. Aijaz, D. Perera, Ronke M. Olabisi
      Pages 79-93

    3. Polymeric Materials for Perm-Selective Coating of Alginate Microbeads

      • William F. Kendall Jr., Emmanuel C. Opara
      Pages 95-109

    4. Determination of the Mechanical Strength of Microcapsules

      • Marcus D. Darabbie, Emmanuel C. Opara
      Pages 111-118

    5. The Diffusive Properties of Hydrogel Microcapsules for Cell Encapsulation

      • D. M. Lavin, B. E. Bintz, C. G. Thanos
      Pages 119-134

    6. Methods for Incorporating Oxygen-Generating Biomaterials into Cell Culture and Microcapsule Systems

      • John Patrick McQuilling, Emmanuel C. Opara
      Pages 135-141

    7. Noninvasive Tracking of Alginate-Microencapsulated Cells

      • Genaro A. Paredes-Juarez, Brad P. Barnett, Jeff W. M. Bulte
      Pages 143-155

    8. Retrieval of Microencapsulated Islet Grafts for Post-transplant Evaluation

      • John Patrick McQuilling, Sivanandane Sittadjody, Rajesh Pareta, Samuel Pendergraft, Clancy J. Clark, Alan C. Farney et al.
      Pages 157-171

  5. Isolation of Cells for Microencapsulation

    1. Front Matter

      Pages 173-173
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    2. A Method of Porcine Pancreatic Islet Isolation for Microencapsulation

      • William F. Kendall Jr., Emmanuel C. Opara
      Pages 175-189

    3. Selective Osmotic Shock (SOS)-Based Islet Isolation for Microencapsulation

      • Kevin Enck, John Patrick McQuilling, Giuseppe Orlando, Riccardo Tamburrini, Sittadjody Sivanandane, Emmanuel C. Opara
      Pages 191-198

    4. Preparation and Characterization of Alginate–Chitosan Microcapsule for Hepatocyte Culture

      • Lanjuan Li, Yanhong Zhang, Xiaoping Pan
      Pages 199-206
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Keywords

About this book

This volume provides a unique forum to review cell microencapsulation in a broad sense by exploring various cell types that have been encapsulated for different purposes, different approaches and devices used for microencapsulation, the biomaterials used in cell microencapsulation, the challenges to the technology, and the current status of its application in different clinical situations. This book is divided in five sections: Section I is an introductory part that discusses historical developments of the technology and its current challenges, as well as the various applications of cell microencapsulation; Section II discusses the main approaches and devices currently used in cell microencapsulation; Section III presents an overview of the various polymeric materials currently in use for cell microencapsulation and the enabling technologies to either monitor or enhance encapsulated cell function; Section IV gives specific examples of the methods used to encapsulate various cell types;and Section V provides an overview of the different clinical situations in which cell microencapsulation has been applied. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and practical, Cell Microencapsulation: Methods and Protocols is a valuable reference for researchers, engineers, clinicians, and other healthcare professionals, as well as food technologists who will find detailed descriptions of methods for the microencapsulation of specific cell types and their current of potential clinical and industrial applications. This volume also includes detailed information about the design and manufacture of different devices including large-scale production devices for use in cell microencapsulation.

Editors and Affiliations

  • Wake Forest Institute for Regenerative Medicine Wake Forest School of Medicine, Winston-Salem, USA

    Emmanuel C. Opara

Bibliographic Information

  • Book Title: Cell Microencapsulation

  • Book Subtitle: Methods and Protocols

  • Editors: Emmanuel C. Opara

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6364-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6362-1Published: 14 October 2016

  • Softcover ISBN: 978-1-4939-8177-9Published: 08 June 2018

  • eBook ISBN: 978-1-4939-6364-5Published: 13 October 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 366

  • Number of Illustrations: 17 b/w illustrations, 80 illustrations in colour

  • Topics: Biotechnology

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