Skip to main content
SpringerLink
Log in

Clostridium difficile

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Adam P. Roberts

    1. Department of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Peter Mullany

    1. Department of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1476)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Restriction Endonuclease Analysis Typing of Clostridium difficile Isolates

    • Susan P. Sambol, Stuart Johnson, Dale N. Gerding
    Pages 1-13

  3. Direct PCR-Ribotyping of Clostridium difficile

    • Sandra Janezic
    Pages 15-21

  4. From FASTQ to Function: In Silico Methods for Processing Next-Generation Sequencing Data

    • Mark D. Preston, Richard A. Stabler
    Pages 23-33

  5. Clostridium difficile Genome Editing Using pyrE Alleles

    • Muhammad Ehsaan, Sarah A. Kuehne, Nigel P. Minton
    Pages 35-52

  6. Use of mCherryOpt Fluorescent Protein in Clostridium difficile

    • Eric M. Ransom, David S. Weiss, Craig D. Ellermeier
    Pages 53-67

  7. A Fluorescent Reporter for Single Cell Analysis of Gene Expression in Clostridium difficile

    • Carolina Piçarra Cassona, Fátima Pereira, Mónica Serrano, Adriano O. Henriques
    Pages 69-90

  8. Clostridium difficile Adhesins

    • Séverine Péchiné, Cécile Denève-Larrazet, Anne Collignon
    Pages 91-101

  9. Intestinal Epithelial Cell Response to Clostridium difficile Flagella

    • Jameel Batah, Imad Kansau
    Pages 103-116

  10. Isolating and Purifying Clostridium difficile Spores

    • Adrianne N. Edwards, Shonna M. McBride
    Pages 117-128

  11. Inducing and Quantifying Clostridium difficile Spore Formation

    • Aimee Shen, Kelly A. Fimlaid, Keyan Pishdadian
    Pages 129-142

  12. Characterization of Functional Prophages in Clostridium difficile

    • Ognjen Sekulović, Louis-Charles Fortier
    Pages 143-165

  13. Induction and Purification of C. difficile Phage Tail-Like Particles

    • John P. Hegarty, William Sangster, Robert E. Ashley, Roland Myers, Susan Hafenstein, David B. Stewart Sr.
    Pages 167-175

  14. Phage Transduction

    • Shan Goh
    Pages 177-185

  15. Transfer of Clostridium difficile Genetic Elements Conferring Resistance to Macrolide–Lincosamide–Streptogramin B (MLSB) Antibiotics

    • Fabrizio Barbanti, François Wasels, Patrizia Spigaglia
    Pages 187-198

  16. Methods for Determining Transfer of Mobile Genetic Elements in Clostridium difficile

    • Priscilla Johanesen, Dena Lyras
    Pages 199-213

  17. Investigating Transfer of Large Chromosomal Regions Containing the Pathogenicity Locus Between Clostridium difficile Strains

    • Michael S. M. Brouwer, Peter Mullany, Elaine Allan, Adam P. Roberts
    Pages 215-222

  18. An In Vitro Model of the Human Colon: Studies of Intestinal Biofilms and Clostridium difficile Infection

    • Grace S. Crowther, Mark H. Wilcox, Caroline H. Chilton
    Pages 223-234

  19. MiniBioReactor Arrays (MBRAs) as a Tool for Studying C. difficile Physiology in the Presence of a Complex Community

    • Jennifer M. Auchtung, Catherine D. Robinson, Kylie Farrell, Robert A. Britton
    Pages 235-258

  20. A Practical Method for Preparation of Fecal Microbiota Transplantation

    • Elizabeth Perez, Christine H. Lee, Elaine O. Petrof
    Pages 259-267
Back to top

Keywords

About this book

This second edition provides 21 new chapters on methods used in laboratories for investigating the physiology and molecular genetics of the pathogen Clostridium difficile. Chapters detail up-to -date experimental techniques for gene editing and transcriptional analysis which are used to investigate the fundamental biology of the organism and its virulence factors.  Additional chapters  describe development of potential new treatments including vaccines, bacteriophage and faecal transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Clostridium difficile: Methods and Protocols, Second Edition provides a comprehensive catalogue of molecular tools and techniques authored by the researchers who have developed them.

Editors and Affiliations

  • Department of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, United Kingdom

    Adam P. Roberts, Peter Mullany

Bibliographic Information

  • Book Title: Clostridium difficile

  • Book Subtitle: Methods and Protocols

  • Editors: Adam P. Roberts, Peter Mullany

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6361-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-6359-1Published: 10 August 2016

  • Softcover ISBN: 978-1-4939-8176-2Published: 09 June 2018

  • eBook ISBN: 978-1-4939-6361-4Published: 09 August 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 292

  • Number of Illustrations: 16 b/w illustrations, 33 illustrations in colour

  • Topics: Immunology

Publish with us

Policies and ethics

Back to top

Search

Navigation