Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1474)
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Table of contents (22 protocols)
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Front Matter
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Molecular Toolbox
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Front Matter
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Fluorescent Microscopy Toolbox
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Front Matter
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Electron Microscopy Toolbox
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Front Matter
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About this book
This volume presents authoritative and cutting-edge methods and protocols focusing on three tool boxes covering the increasingly diverse methodologies used to image selected proteins and to investigate their function by light and electron microscopy. The first tool box includes the development of a wide range of molecular and immunological probes to target specific proteins. The second details the use of these probes for high resolution fluorescence microscopy and the third focuses on applications for transmission and scanning electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls thus ensuring successful results in the further study of this vital field.
Reviews
Editors and Affiliations
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Department of Biological Sciences, The University of Memphis, Memphis, USA
Steven D. Schwartzbach, Omar Skalli
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Department of Anatomy, Universidad Central del Caribe, Bayamon, USA
Thomas Schikorski
Bibliographic Information
Book Title: High-Resolution Imaging of Cellular Proteins
Book Subtitle: Methods and Protocols
Editors: Steven D. Schwartzbach, Omar Skalli, Thomas Schikorski
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6352-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-6350-8Published: 12 August 2016
Softcover ISBN: 978-1-4939-8173-1Published: 09 June 2018
eBook ISBN: 978-1-4939-6352-2Published: 11 August 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 366
Number of Illustrations: 24 b/w illustrations, 21 illustrations in colour
Topics: Imaging / Radiology