Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1470)
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Table of contents (19 protocols)
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Front Matter
About this book
High-throughput RNAi screening remains one of the most widely used technologies to perform target identification and validation studies in an unbiased manner. These assays are equally important for research and development across academic, biotech, and pharmaceutical industries. The success of these screening efforts is dependent on robust methodologies to perform these screens. In High-Throughput RNAi Screening: Methods and Protocols, expert researchers in the field share protocols and methods for performing high-throughput RNAi (HT-RNAi) screens. These include the use of various RNAi platforms and delivery methods in mammalian and non-mammalian systems, whole organism and cell models, and various applications, such as drug sensitizer identification. Finally, the book examines the latest advancements in the fields of assay development, library screening, data analysis, and hit selection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, High-Throughput RNAi Screening: Methods and Protocols provides a comprehensive source of protocols and other necessary information to make robust and successful assays possible for all who wish to apply HT-RNAi in their research.
Editors and Affiliations
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Institute of Molecular Medicine, Phoenix Children’s Hospital, Phoenix, USA
David O. Azorsa
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Constellation Pharmaceuticals, Cambridge, USA
Shilpi Arora
Bibliographic Information
Book Title: High-Throughput RNAi Screening
Book Subtitle: Methods and Protocols
Editors: David O. Azorsa, Shilpi Arora
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6337-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-6335-5Published: 31 August 2016
Softcover ISBN: 978-1-4939-8168-7Published: 09 June 2018
eBook ISBN: 978-1-4939-6337-9Published: 31 August 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 262
Number of Illustrations: 9 b/w illustrations, 43 illustrations in colour
Topics: Human Genetics