Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1469)
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Table of contents (17 protocols)
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Front Matter
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Back Matter
About this book
This volume assembles protocols for chromosome engineering and genome editing in two recently developed approaches for manipulating chromosomal and genomic DNA in plants. The first approach is a “plant chromosome vector” system, which allows the introduction of desired genes or DNA into target sites on the chromosome vector, particularly by sequence-specific recombination. The second approach is “genome-editing,” which makes it possible to introduce mutations into any of the genes of DNA that we wish to change. In addition, this book also covers other related techniques used to accelerate progress in plant chromosome and genome engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Chromosome and Genomic Engineering in Plants: Methods and Protocols provides a comprehensive source of protocols and other necessary information to anyone interested in this field of study.
Editors and Affiliations
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Institute of Plant Science and Resources, Okayama University, Kurashiki, Japan
Minoru Murata
Bibliographic Information
Book Title: Chromosome and Genomic Engineering in Plants
Book Subtitle: Methods and Protocols
Editors: Minoru Murata
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-4931-1
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-4929-8Published: 25 August 2016
Softcover ISBN: 978-1-4939-8163-2Published: 21 April 2018
eBook ISBN: 978-1-4939-4931-1Published: 24 August 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 218
Number of Illustrations: 24 b/w illustrations, 38 illustrations in colour
Topics: Plant Sciences