Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains keynotes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1467)
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Table of contents (19 protocols)
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Front Matter
About this book
This volume provides a thorough overview of the Wilms’ Tumour Gene (WT1). The book begins with three review chapters that cover the involvement of WT1 in pediatric cancer, kidney disease, and tissue development and homeostasis. The next few chapters discuss cell marking and lineage tracing, epicardial cell methodology, colony forming assays for bone marrow stem cells, angiogenesis assays and zebrafish tools. The next group of chapters explores the latest tools in genomics, molecular biology, and biochemistry. They discuss dissecting transcription factor function in cell free systems, ChiP seq, proteomics, RNA interactome, and multiphoton imaging of lipids, measuring the binding constants of protein-nucleic acid interactions, and bioinformatics approaches for analyzing Next Generation Sequence data. The final chapter discusses protocols for clinical trials for immune therapy using anti-WT1 peptides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and thorough, The Wilms’ Tumour (WT1) Gene: Methods and Protocols is a valuable resource for anyone who is interested in the diverse methodologies used in WT1 research.
Editors and Affiliations
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MRC Human Genetics Unit, University of Edinburgh, Edinburgh, United Kingdom
Nicholas Hastie
Bibliographic Information
Book Title: The Wilms' Tumor (WT1) Gene
Book Subtitle: Methods and Protocols
Editors: Nicholas Hastie
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-4023-3
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-4021-9Published: 20 July 2016
Softcover ISBN: 978-1-4939-8156-4Published: 30 May 2018
eBook ISBN: 978-1-4939-4023-3Published: 14 July 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 235
Number of Illustrations: 8 b/w illustrations, 34 illustrations in colour
Topics: Cancer Research, Human Genetics