Capillary Electrophoresis of Proteins and Peptides

1. Front Matter

   Pages i-xi

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2. Chemical and Instrumental Approaches for Capillary Electrophoresis (CE)–Fluorescence Analysis of Proteins

   • Isabelle Le Potier, Audrey Boutonnet, Vincent Ecochard, François Couderc

   Pages 1-10

3. Discrimination of Glycoproteins from Unglycosylated Proteins in Capillary Electrophoresis: Two-Color LIF Detection Coupled with Post-column Derivatization

   • Takashi Kaneta

   Pages 11-23

4. Capillary Zone Electrophoresis–Mass Spectrometry of Intact Proteins

   • Elena Domínguez-Vega, Rob Haselberg, Govert W. Somsen

   Pages 25-41

5. Screening of Small Intact Proteins by Capillary Electrophoresis Electrospray Ionization-Mass Spectrometry (CE-ESI-MS)

   • Sabine Neuberger, Angelina Rafai, Christian Neusüß

   Pages 43-56

6. Online Capillary IsoElectric Focusing-ElectroSpray Ionization Mass Spectrometry (CIEF-ESI MS) in Glycerol–Water Media for the Separation and Characterization of Hydrophilic and Hydrophobic Proteins

   • Meriem Mokaddem, Fanny d’Orlyé, Anne Varenne

   Pages 57-66

7. On-Line Solid-Phase Extraction Capillary Electrophoresis Mass Spectrometry for Preconcentration and Clean-Up of Peptides and Proteins

   • Fernando Benavente, Silvia Medina-Casanellas, Estela Giménez, Victoria Sanz-Nebot

   Pages 67-84

8. Affinity Monolith-Integrated Microchips for Protein Purification and Concentration

   • Changlu Gao, Xiuhua Sun, Huaixin Wang, Wei Qiao, Bo Hu

   Pages 85-92

9. Analysis of Somatropin by Double-Injection Capillary-Zone Electrophoresis in Polybrene/Chondroitin Sulfate A Double-Coated Capillaries

   • Ahmad Amini

   Pages 93-105

10. Poly(N,N-Dimethylacrylamide)-Based Coatings to Modulate Electroosmotic Flow and Capillary Surface Properties for Protein Analysis

    • Laura Sola, Marina Cretich, Marcella Chiari

    Pages 107-119

11. Measurement of Inflammatory Chemokines in Micro-dissected Tissue Biopsy Samples by Chip-Based Immunoaffinity Capillary Electrophoresis

    • Terry M. Phillips, Edward Wellner, Shane McMohan, Heather Kalish

    Pages 121-136

12. Separation of Recombinant Therapeutic Proteins Using Capillary Gel Electrophoresis and Capillary Isoelectric Focusing

    • Caitlyn A. G. De Jong, Jessica Risley, Alexis K. Lee, Shuai Sherry Zhao, David D. Y. Chen

    Pages 137-149

13. Characterization of Chemical and Physical Modifications of Human Serum Albumin by Capillary Zone Electrophoresis

    • Anne-Lise Marie, Nguyet Thuy Tran, Myriam Taverna

    Pages 151-163

14. Capillary Electrophoresis Method for the Assessment of Erythropoiesis-Stimulating Agents in Final Formulations

    • Michel Girard, Anita Kane, Sylvie Boucher

    Pages 165-177

15. Quality Control of Therapeutic Monoclonal Antibodies at the Hospital After Their Compounding and Before Their Administration to Patients

    • Emmanuel Jaccoulet, Claire Smadja, Myriam Taverna

    Pages 179-184

16. Capillary Electrophoresis-Ultraviolet-Mass Spectrometry (CE-UV-MS) for the Simultaneous Determination and Quantification of Insulin Formulations

    • Julie Schappler, Serge Rudaz

    Pages 185-195

17. Applications of an Automated and Quantitative CE-Based Size and Charge Western Blot for Therapeutic Proteins and Vaccines

    • Richard R. Rustandi, Melissa Hamm, Catherine Lancaster, John W. Loughney

    Pages 197-217

18. Affinity Capillary Electrophoresis Applied to Investigation of Valinomycin Complexes with Ammonium and Alkali Metal Ions

    • Sille Štěpánová, Václav Kašička

    Pages 219-232

19. Back Matter

    Pages 233-234

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This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins. With practical detail, the contents cover different modes of capillary electrophoresis (CE) useful for protein and peptide analysis, CZE, CIEF, ACE, CGE, and different types of application such as the quality control of therapeutic proteins and monoclonal antibodies, clinical analyses of chemokines in tissues, qualitative and quantitative analysis of vaccine proteins, and determination of binding constants in complexes involving peptides or proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and exhaustive, Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols serves both beginners and experts with a collection of the current and most active topics in this vital field of study.

• CE-MS
• Intact proteins
• Detection methods
• Therapeutic proteins
• Quality control
• Vaccine proteins
• Glycoprotein analysis

• Institut Galien Paris-Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Université Paris-Saclay, Châtenay-Malabry, France

  Nguyet Thuy Tran, Myriam Taverna

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