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Tumor Angiogenesis Assays

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Domenico Ribatti

    1. Neurosciences and Sensory Organs, University of Bari,School of Med Dept of Basic Med Sci, Bari, Italy

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1464)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. The Discovery of Tumor Angiogenesis Factors: A Historical Overview

    • Domenico Ribatti
    Pages 1-12

  3. Morphological Aspects of Tumor Angiogenesis

    • Ruslan Hlushchuk, Sébastien Barré, Valentin Djonov
    Pages 13-24

  4. Exosomes in Tumor Angiogenesis

    • Karma Z. Salem, Michele Moschetta, Antonio Sacco, Luisa Imberti, Giuseppe Rossi, Irene M. Ghobrial et al.
    Pages 25-34

  5. Coculture Assays for Endothelial Cells-Mural Cells Interactions

    • Diana M. Sánchez-Palencia, Alex Bigger-Allen, Magali Saint-Geniez, Joseph F. Arboleda-Velásquez, Patricia A. D’Amore
    Pages 35-47

  6. Characterization of Cancer Stem Cells

    • Stefania Orecchioni, Francesco Bertolini
    Pages 49-62

  7. The Aortic Ring Assay and Its Use for the Study of Tumor Angiogenesis

    • Alfred C. Aplin, Roberto F. Nicosia
    Pages 63-72

  8. Surface Plasmon Resonance Analysis of Heparin-Binding Angiogenic Growth Factors

    • Marco Rusnati, Antonella Bugatti
    Pages 73-84

  9. An Ex Vivo Tissue Culture Model for Anti-angiogenic Drug Testing

    • Mohammad S. Azimi, Michelle Lacey, Debasis Mondal, Walter L. Murfee
    Pages 85-95

  10. The Chorioallantoic Membrane of the Chick Embryo to Assess Tumor Formation and Metastasis

    • Anne Herrmann, Diana Moss, Violaine Sée
    Pages 97-105

  11. Transgenic Zebrafish

    • Xiaowen Chen, Dafne Gays, Massimo M. Santoro
    Pages 107-114

  12. High-Resolution Intravital Microscopy of Tumor Angiogenesis

    • Ann L. B. Seynhaeve, Timo L. M. ten Hagen
    Pages 115-127

  13. Subcutaneous Murine Xenograft Models: A Critical Tool for Studying Human Tumor Growth and Angiogenesis In Vivo

    • Katharina M. Schmidt, Edward K. Geissler, Sven A. Lang
    Pages 129-137

  14. Orthotopic Model of Ovarian Cancer

    • Alessandra Decio, Raffaella Giavazzi
    Pages 139-149

  15. The Rip1Tag2 Transgenic Mouse Model

    • Ruben Bill, Gerhard Christofori
    Pages 151-161

  16. Tumor Blood Vessel Visualization

    • Clarissa Gillmann, Tobias Bäuerle
    Pages 163-173

  17. Uncovering Metabolic Effects of Anti-angiogenic Therapy in Tumors by Induced Metabolic Bioluminescence Imaging

    • Stefano Indraccolo, Stefan Walenta, Wolfgang Mueller-Klieser
    Pages 175-184

  18. Quantitation of Tumor Angiogenesis In Vitro: An All-In-One Angiogenesis Assay

    • Mahtab Bahramsoltani, Ward De Spiegelaere
    Pages 185-191

  19. Back Matter

    Pages 193-194
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Keywords

About this book

This volume aims to provide a range of methods and protocols for studying tumor angiogenesis in vitro and in vivo to reflect advances in the field. The chapters in this book cover topics such as: morphological aspects of tumor angiogenesis, aortic ring assay and its use for the study of tumor angiogenesis, ex vivo tissue culture model for anti-angiogenic drug testing, transgenic zebrafish, orthotopic models of ovarian cancer, and uncovering metabolic effects of anti-angiogenic therapy in tumors by induced metabolic bioluminescence imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, Tumor Angiogenesis Assays: Methods and Protocols is a valuable resource for anyone interested in tumor angiogenesis assay research.

 

Editors and Affiliations

  • Neurosciences and Sensory Organs, University of Bari,School of Med Dept of Basic Med Sci, Bari, Italy

    Domenico Ribatti

Bibliographic Information

  • Book Title: Tumor Angiogenesis Assays

  • Book Subtitle: Methods and Protocols

  • Editors: Domenico Ribatti

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3999-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3997-8Published: 17 November 2016

  • Softcover ISBN: 978-1-4939-8149-6Published: 27 June 2018

  • eBook ISBN: 978-1-4939-3999-2Published: 16 November 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 194

  • Number of Illustrations: 17 b/w illustrations, 40 illustrations in colour

  • Topics: Cancer Research

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