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Unconventional Protein Secretion

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Andrea Pompa

    1. Instituto di Bioscienze e Biorisorse, Perugia, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Francesca De Marchis

    1. Instituto di Bioscienze e Biorisorse, Perugia, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1459)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Background

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. ER to Golgi-Dependent Protein Secretion: The Conventional Pathway

      • Corrado Viotti
      Pages 3-29

    3. Unconventional Protein Secretion in Animal Cells

      • Fanny Ng, Bor Luen Tang
      Pages 31-46

    4. Unconventional Protein Secretion in Plants

      • Destiny J. Davis, Byung-Ho Kang, Angelo S. Heringer, Thomas E. Wilkop, Georgia Drakakaki
      Pages 47-63

  3. UPS Contemplates Multidisciplinary Approaches for Plants

    1. Front Matter

      Pages 65-65
      Download chapter PDF

    2. Chemical Secretory Pathway Modulation in Plant Protoplasts

      • Francesca De Marchis, Andrea Pompa, Michele Bellucci
      Pages 67-79

    3. From Cytosol to the Apoplast: The Hygromycin Phosphotransferase (HYGR) Model in Arabidopsis

      • Haiyan Zhang, Jinjin Li
      Pages 81-90

    4. Following the Time-Course of Post-pollination Events by Transmission Electron Microscopy (TEM): Buildup of Exosome-Like Structures with Compatible Pollinations

      • Darya Safavian, Jennifer Doucet, Daphne R. Goring
      Pages 91-101

  4. UPS Contemplates Multidisciplinary Approaches for Animals and Fungi

    1. Front Matter

      Pages 103-103
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    2. Investigating Alternative Transport of Integral Plasma Membrane Proteins from the ER to the Golgi: Lessons from the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)

      • Margarida D. Amaral, Carlos M. Farinha, Paulo Matos, Hugo M. Botelho
      Pages 105-126

    3. Quantification of a Non-conventional Protein Secretion: The Low-Molecular-Weight FGF-2 Example

      • Tania Arcondéguy, Christian Touriol, Eric Lacazette
      Pages 127-134

    4. Human Primary Keratinocytes as a Tool for the Analysis of Caspase-1-Dependent Unconventional Protein Secretion

      • Gerhard E. Strittmatter, Martha Garstkiewicz, Jennifer Sand, Serena Grossi, Hans-Dietmar Beer
      Pages 135-147

    5. A Reporter System to Study Unconventional Secretion of Proteins Avoiding N-Glycosylation in Ustilago maydis

      • Janpeter Stock, Marius Terfrüchte, Kerstin Schipper
      Pages 149-160

    6. Stress-Inducible Protein 1 (STI1): Extracellular Vesicle Analysis and Quantification

      • Marcos Vinicios Salles Dias, Vilma Regina Martins, Glaucia Noeli Maroso Hajj
      Pages 161-174

    7. Analysis of Yeast Extracellular Vesicles

      • Marcio L. Rodrigues, Debora L. Oliveira, Gabriele Vargas, Wendell Girard-Dias, Anderson J. Franzen, Susana Frasés et al.
      Pages 175-190

    8. Exploring the Leishmania Hydrophilic Acylated Surface Protein B (HASPB) Export Pathway by Live Cell Imaging Methods

      • Lorna MacLean, Helen Price, Peter O’Toole
      Pages 191-203

    9. Characterization of the Unconventional Secretion of the Ebola Matrix Protein VP40

      • Olivier Reynard, Mathieu Mateo
      Pages 205-213

    10. Role and Characterization of Synuclein-γ Unconventional Protein Secretion in Cancer Cells

      • Caiyun Liu, Like Qu, Chengchao Shou
      Pages 215-227

  5. Secretome Isolation from Plant and Animal Samples to Identify Leaderless Secretory Proteins (LSP)

    1. Front Matter

      Pages 229-229
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Keywords

About this book

This volume presents relevant background information to understanding the molecular basis governing unconventional protein secretion (UPS), and in particular explores the latest techniques and protocols that have been successfully applied for the study of this topic. Detailed chapters include an overview of conventional and unconventional secretory pathways along with multidisciplinary approaches and methods used for UPS analysis in different organisms. Written in the highly successful Methods in Molecular Biology series format,chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Unconventional Protein Secretion: Methods and Protocols will beuseful for all interested in the secretory pathway field as well as applications in cell biology, cell development, biomedical research, and healthcare.


Editors and Affiliations

  • Instituto di Bioscienze e Biorisorse, Perugia, Italy

    Andrea Pompa, Francesca De Marchis

Bibliographic Information

  • Book Title: Unconventional Protein Secretion

  • Book Subtitle: Methods and Protocols

  • Editors: Andrea Pompa, Francesca De Marchis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3804-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3802-5Published: 24 September 2016

  • Softcover ISBN: 978-1-4939-8144-1Published: 14 June 2018

  • eBook ISBN: 978-1-4939-3804-9Published: 24 September 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 273

  • Number of Illustrations: 15 b/w illustrations, 28 illustrations in colour

  • Topics: Protein Science

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