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  • © 2016

Unconventional Protein Secretion

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1459)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiv
  2. Background

    1. Front Matter

      Pages 1-1
    2. Unconventional Protein Secretion in Animal Cells

      • Fanny Ng, Bor Luen Tang
      Pages 31-46
    3. Unconventional Protein Secretion in Plants

      • Destiny J. Davis, Byung-Ho Kang, Angelo S. Heringer, Thomas E. Wilkop, Georgia Drakakaki
      Pages 47-63
  3. UPS Contemplates Multidisciplinary Approaches for Plants

    1. Front Matter

      Pages 65-65
    2. Chemical Secretory Pathway Modulation in Plant Protoplasts

      • Francesca De Marchis, Andrea Pompa, Michele Bellucci
      Pages 67-79
  4. UPS Contemplates Multidisciplinary Approaches for Animals and Fungi

    1. Front Matter

      Pages 103-103
    2. Quantification of a Non-conventional Protein Secretion: The Low-Molecular-Weight FGF-2 Example

      • Tania Arcondéguy, Christian Touriol, Eric Lacazette
      Pages 127-134
    3. Human Primary Keratinocytes as a Tool for the Analysis of Caspase-1-Dependent Unconventional Protein Secretion

      • Gerhard E. Strittmatter, Martha Garstkiewicz, Jennifer Sand, Serena Grossi, Hans-Dietmar Beer
      Pages 135-147
    4. Stress-Inducible Protein 1 (STI1): Extracellular Vesicle Analysis and Quantification

      • Marcos Vinicios Salles Dias, Vilma Regina Martins, Glaucia Noeli Maroso Hajj
      Pages 161-174
    5. Analysis of Yeast Extracellular Vesicles

      • Marcio L. Rodrigues, Debora L. Oliveira, Gabriele Vargas, Wendell Girard-Dias, Anderson J. Franzen, Susana Frasés et al.
      Pages 175-190
  5. Secretome Isolation from Plant and Animal Samples to Identify Leaderless Secretory Proteins (LSP)

    1. Front Matter

      Pages 229-229

About this book

This volume presents relevant background information to understanding the molecular basis governing unconventional protein secretion (UPS), and in particular explores the latest techniques and protocols that have been successfully applied for the study of this topic. Detailed chapters include an overview of conventional and unconventional secretory pathways along with multidisciplinary approaches and methods used for UPS analysis in different organisms. Written in the highly successful Methods in Molecular Biology series format,chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Unconventional Protein Secretion: Methods and Protocols will beuseful for all interested in the secretory pathway field as well as applications in cell biology, cell development, biomedical research, and healthcare.


Editors and Affiliations

  • Instituto di Bioscienze e Biorisorse, Perugia, Italy

    Andrea Pompa, Francesca De Marchis

Bibliographic Information

  • Book Title: Unconventional Protein Secretion

  • Book Subtitle: Methods and Protocols

  • Editors: Andrea Pompa, Francesca De Marchis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3804-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3802-5Published: 24 September 2016

  • Softcover ISBN: 978-1-4939-8144-1Published: 14 June 2018

  • eBook ISBN: 978-1-4939-3804-9Published: 24 September 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 273

  • Number of Illustrations: 15 b/w illustrations, 28 illustrations in colour

  • Topics: Protein Science

Buy it now

Buying options

eBook USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 54.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access