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The Tumor Microenvironment

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Josie Ursini-Siegel

    1. Lady Davis Institute for Medical Research, Montreal, Canada

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    You can also search for this editor in PubMed   Google Scholar

  2. Nicole Beauchemin

    1. Goodman Cancer Research Centre, McGill University, Montreal, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols for tumor microenvironment research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1458)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Methods of Immunohistochemistry and Immunofluorescence: Converting Invisible to Visible

    • Hidetoshi Mori, Robert D. Cardiff
    Pages 1-12

  3. Laser Capture Microdissection as a Tool to Study Tumor Stroma

    • Nicholas R. Bertos, Morag Park
    Pages 13-25

  4. Quantitative Analysis of Human Cancer Cell Extravasation Using Intravital Imaging

    • Lian Willetts, David Bond, Konstantin Stoletov, John D. Lewis
    Pages 27-37

  5. Studies on the Tumor Vasculature and Coagulant Microenvironment

    • Esterina D’Asti, Brian Meehan, Janusz Rak
    Pages 39-58

  6. A Microfluidic Method to Mimic Luminal Structures in the Tumor Microenvironment

    • José A. Jiménez-Torres, David J. Beebe, Kyung E. Sung
    Pages 59-69

  7. Measuring Vascular Permeability In Vivo

    • Eelco F. J. Meijer, James W. Baish, Timothy P. Padera, Dai Fukumura
    Pages 71-85

  8. Hydroxylation-Dependent Interaction of Substrates to the Von Hippel-Lindau Tumor Suppressor Protein (VHL)

    • Pardeep Heir, Michael Ohh
    Pages 87-94

  9. Analyzing the Tumor Microenvironment by Flow Cytometry

    • Yoon Kow Young, Alicia M. Bolt, Ryuhjin Ahn, Koren K. Mann
    Pages 95-110

  10. Detecting Secreted Analytes from Immune Cells: An Overview of Technologies

    • Kelly A. Pike, Caitlyn Hui, Connie M. Krawczyk
    Pages 111-124

  11. Purification of Immune Cell Populations from Freshly Isolated Murine Tumors and Organs by Consecutive Magnetic Cell Sorting and Multi-parameter Flow Cytometry-Based Sorting

    • Camilla Salvagno, Karin E. de Visser
    Pages 125-135

  12. Viral Engineering of Chimeric Antigen Receptor Expression on Murine and Human T Lymphocytes

    • Joanne A. Hammill, Arya Afsahi, Jonathan L. Bramson, Christopher W. Helsen
    Pages 137-157

  13. Methods to Evaluate the Antitumor Activity of Immune Checkpoint Inhibitors in Preclinical Studies

    • Bertrand Allard, David Allard, John Stagg
    Pages 159-177

  14. Isolation and Characterization of Low- vs. High-Density Neutrophils in Cancer

    • Jitka Y. Sagiv, Sandra Voels, Zvi Granot
    Pages 179-193

  15. Analysis of Extracellular Vesicles in the Tumor Microenvironment

    • Khalid Al-Nedawi, Jolene Read
    Pages 195-202

  16. Visualizing the Tumor Microenvironment of Liver Metastasis by Spinning Disk Confocal Microscopy

    • Liane Babes, Paul Kubes
    Pages 203-215

  17. Intravital Microscopy for Imaging the Tumor Microenvironment in Live Mice

    • Victor Naumenko, Craig Jenne, Douglas J. Mahoney
    Pages 217-230

  18. Development of a Patient-Derived Xenograft Model Using Brain Tumor Stem Cell Systems to Study Cancer

    • Chirayu Chokshi, Manvir Dhillon, Nicole McFarlane, Chitra Venugopal, Sheila K. Singh
    Pages 231-245

  19. Modeling Breast Tumor Development with a Humanized Mouse Model

    • Lisa M. Arendt
    Pages 247-259

  20. CRISPR/Cas9 Genome Editing as a Strategy to Study the Tumor Microenvironment in Transgenic Mice

    • Yojiro Yamanaka
    Pages 261-271
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Keywords

About this book

This book covers core and emerging in vitro and in vivo protocols used to study how various components of the tumor microenvironment are established and subsequently interact with tumor cells to facilitate carcinogenesis. In addition, the book examines research topics including cellular and molecular biology approaches, in vivo genetic approaches, various “omics”-based strategies, therapeutic strategies to target the microenvironment, and, finally, advanced techniques in the fields of tissue engineering and nanotechnology.  Written and validated in the laboratories of a number of trusted collaborating authors for the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, The Tumor Microenvironment: Methods and Protocols constitutes a compendium of techniques now available to a broad audience, including basic and clinician scientists, systems biologists, and biological engineers.

Editors and Affiliations

  • Lady Davis Institute for Medical Research, Montreal, Canada

    Josie Ursini-Siegel

  • Goodman Cancer Research Centre, McGill University, Montreal, Canada

    Nicole Beauchemin

Bibliographic Information

  • Book Title: The Tumor Microenvironment

  • Book Subtitle: Methods and Protocols

  • Editors: Josie Ursini-Siegel, Nicole Beauchemin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3801-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3799-8Published: 01 September 2016

  • Softcover ISBN: 978-1-4939-8143-4Published: 09 June 2018

  • eBook ISBN: 978-1-4939-3801-8Published: 31 August 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 356

  • Number of Illustrations: 19 b/w illustrations, 19 illustrations in colour

  • Topics: Cancer Research

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