The Tumor Microenvironment

1. Front Matter

   Pages i-xii

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2. Methods of Immunohistochemistry and Immunofluorescence: Converting Invisible to Visible

   • Hidetoshi Mori, Robert D. Cardiff

   Pages 1-12

3. Laser Capture Microdissection as a Tool to Study Tumor Stroma

   • Nicholas R. Bertos, Morag Park

   Pages 13-25

4. Quantitative Analysis of Human Cancer Cell Extravasation Using Intravital Imaging

   • Lian Willetts, David Bond, Konstantin Stoletov, John D. Lewis

   Pages 27-37

5. Studies on the Tumor Vasculature and Coagulant Microenvironment

   • Esterina D’Asti, Brian Meehan, Janusz Rak

   Pages 39-58

6. A Microfluidic Method to Mimic Luminal Structures in the Tumor Microenvironment

   • José A. Jiménez-Torres, David J. Beebe, Kyung E. Sung

   Pages 59-69

7. Measuring Vascular Permeability In Vivo

   • Eelco F. J. Meijer, James W. Baish, Timothy P. Padera, Dai Fukumura

   Pages 71-85

8. Hydroxylation-Dependent Interaction of Substrates to the Von Hippel-Lindau Tumor Suppressor Protein (VHL)

   • Pardeep Heir, Michael Ohh

   Pages 87-94

9. Analyzing the Tumor Microenvironment by Flow Cytometry

   • Yoon Kow Young, Alicia M. Bolt, Ryuhjin Ahn, Koren K. Mann

   Pages 95-110

10. Detecting Secreted Analytes from Immune Cells: An Overview of Technologies

    • Kelly A. Pike, Caitlyn Hui, Connie M. Krawczyk

    Pages 111-124

11. Purification of Immune Cell Populations from Freshly Isolated Murine Tumors and Organs by Consecutive Magnetic Cell Sorting and Multi-parameter Flow Cytometry-Based Sorting

    • Camilla Salvagno, Karin E. de Visser

    Pages 125-135

12. Viral Engineering of Chimeric Antigen Receptor Expression on Murine and Human T Lymphocytes

    • Joanne A. Hammill, Arya Afsahi, Jonathan L. Bramson, Christopher W. Helsen

    Pages 137-157

13. Methods to Evaluate the Antitumor Activity of Immune Checkpoint Inhibitors in Preclinical Studies

    • Bertrand Allard, David Allard, John Stagg

    Pages 159-177

14. Isolation and Characterization of Low- vs. High-Density Neutrophils in Cancer

    • Jitka Y. Sagiv, Sandra Voels, Zvi Granot

    Pages 179-193

15. Analysis of Extracellular Vesicles in the Tumor Microenvironment

    • Khalid Al-Nedawi, Jolene Read

    Pages 195-202

16. Visualizing the Tumor Microenvironment of Liver Metastasis by Spinning Disk Confocal Microscopy

    • Liane Babes, Paul Kubes

    Pages 203-215

17. Intravital Microscopy for Imaging the Tumor Microenvironment in Live Mice

    • Victor Naumenko, Craig Jenne, Douglas J. Mahoney

    Pages 217-230

18. Development of a Patient-Derived Xenograft Model Using Brain Tumor Stem Cell Systems to Study Cancer

    • Chirayu Chokshi, Manvir Dhillon, Nicole McFarlane, Chitra Venugopal, Sheila K. Singh

    Pages 231-245

19. Modeling Breast Tumor Development with a Humanized Mouse Model

    • Lisa M. Arendt

    Pages 247-259

20. CRISPR/Cas9 Genome Editing as a Strategy to Study the Tumor Microenvironment in Transgenic Mice

    • Yojiro Yamanaka

    Pages 261-271

This book covers core and emerging in vitro and in vivo protocols used to study how various components of the tumor microenvironment are established and subsequently interact with tumor cells to facilitate carcinogenesis. In addition, the book examines research topics including cellular and molecular biology approaches, in vivo genetic approaches, various “omics”-based strategies, therapeutic strategies to target the microenvironment, and, finally, advanced techniques in the fields of tissue engineering and nanotechnology.  Written and validated in the laboratories of a number of trusted collaborating authors for the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, The Tumor Microenvironment: Methods and Protocols constitutes a compendium of techniques now available to a broad audience, including basic and clinician scientists, systems biologists, and biological engineers.

• Tumor cells
• Carcinogenesis
• Therapeutic strategies
• Genetic approaches
• Cancer research
• Extracellular matrix

• Lady Davis Institute for Medical Research, Montreal, Canada

  Josie Ursini-Siegel

• Goodman Cancer Research Centre, McGill University, Montreal, Canada

  Nicole Beauchemin

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