Skip to main content
SpringerLink
Log in
Book cover

Proteostasis

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Rune Matthiesen

    1. Computational and Experimental Biology Group Department of Health Promotion and Chronic Diseases, National Health Institute Dr. Ricardo Jorge, INSA, I.P., Lisboa, Portugal

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1449)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (31 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. UPS Activation in the Battle Against Aging and Aggregation-Related Diseases: An Extended Review

    • Nikoletta Papaevgeniou, Niki Chondrogianni
    Pages 1-70

  3. Review and Literature Mining on Proteostasis Factors and Cancer

    • Ana Sofia Carvalho, Manuel S. Rodríguez, Rune Matthiesen
    Pages 71-84

  4. Combining Zebrafish and Mouse Models to Test the Function of Deubiquitinating Enzyme (Dubs) Genes in Development: Role of USP45 in the Retina

    • Vasileios Toulis, Alejandro Garanto, Gemma Marfany
    Pages 85-101

  5. Immunodepletion and Immunopurification as Approaches for CSN Research

    • Amnon Golan, Ning Wei, Elah Pick
    Pages 103-116

  6. Studying Protein Ubiquitylation in Yeast

    • Junie Hovsepian, Michel Becuwe, Oded Kleifeld, Michael H. Glickman, Sébastien Léon
    Pages 117-142

  7. Strategies to Detect Endogenous Ubiquitination of a Target Mammalian Protein

    • Sara Sigismund, Simona Polo
    Pages 143-151

  8. In Vitro Ubiquitination: Self-Ubiquitination, Chain Formation, and Substrate Ubiquitination Assays

    • Elena Maspero, Simona Polo
    Pages 153-160

  9. Isolation of the Ubiquitin-Proteome from Tumor Cell Lines and Primary Cells Using TUBEs

    • Wendy Xolalpa, Lydia Mata-Cantero, Fabienne Aillet, Manuel S. Rodriguez
    Pages 161-175

  10. TUBEs-Mass Spectrometry for Identification and Analysis of the Ubiquitin-Proteome

    • Mikel Azkargorta, Iraide Escobes, Felix Elortza, Rune Matthiesen, Manuel S. Rodríguez
    Pages 177-192

  11. Isolation of Ubiquitinated Proteins to High Purity from In Vivo Samples

    • Juanma Ramirez, Mingwei Min, Rosa Barrio, Catherine Lindon, Ugo Mayor
    Pages 193-202

  12. Method for the Purification of Endogenous Unanchored Polyubiquitin Chains

    • Daniel Scott, Jo Strachan, Varun Gopala Krishna, Barry Shaw, David J. Tooth, Mark S. Searle et al.
    Pages 203-213

  13. Fluorescent Tools for In Vivo Studies on the Ubiquitin-Proteasome System

    • Olli Matilainen, Sweta Jha, Carina I. Holmberg
    Pages 215-222

  14. Bimolecular Fluorescence Complementation to Assay the Interactions of Ubiquitylation Enzymes in Living Yeast Cells

    • Ewa Blaszczak, Claude Prigent, Gwenaël Rabut
    Pages 223-241

  15. Monitoring Ubiquitin-Coated Bacteria via Confocal Microscopy

    • Marie Lork, Mieke Delvaeye, Amanda Gonçalves, Evelien Van Hamme, Rudi Beyaert
    Pages 243-250

  16. Detection and Analysis of Cell Cycle-Associated APC/C-Mediated Cellular Ubiquitylation In Vitro and In Vivo

    • Cesyen Cedeño, Esther La Monaca, Mara Esposito, Gustavo J. Gutierrez
    Pages 251-265

  17. Detection and Analysis of SUMOylation Substrates In Vitro and In Vivo

    • Cesyen Cedeño, Esther La Monaca, Mara Esposito, Gustavo J. Gutierrez
    Pages 267-278

  18. Detection of Protein–Protein Interactions and Posttranslational Modifications Using the Proximity Ligation Assay: Application to the Study of the SUMO Pathway

    • Marko Ristic, Frédérique Brockly, Marc Piechaczyk, Guillaume Bossis
    Pages 279-290

  19. Dissecting SUMO Dynamics by Mass Spectrometry

    • Krzysztof Drabikowski, MichaÅ‚ Dadlez
    Pages 291-298

  20. Isolation of Lysosomes from Mammalian Tissues and Cultured Cells

    • Carmen Aguado, Eva Pérez-Jiménez, Marcos Lahuerta, Erwin Knecht
    Pages 299-311
Back to top

Keywords

About this book

This volume highlights the role of proteostasis in human health and associated disease model systems, reflecting its rising importance which has led to the development of new technologies to obtain insight into underling protein mechanistic events. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Proteostasis: Methods and Protocols aims to become a reference book on proteostasis in human health.



Editors and Affiliations

  • Computational and Experimental Biology Group Department of Health Promotion and Chronic Diseases, National Health Institute Dr. Ricardo Jorge, INSA, I.P., Lisboa, Portugal

    Rune Matthiesen

Bibliographic Information

  • Book Title: Proteostasis

  • Book Subtitle: Methods and Protocols

  • Editors: Rune Matthiesen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3756-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3754-7Published: 10 September 2016

  • Softcover ISBN: 978-1-4939-8130-4Published: 09 June 2018

  • eBook ISBN: 978-1-4939-3756-1Published: 09 September 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 488

  • Number of Illustrations: 34 b/w illustrations, 60 illustrations in colour

  • Topics: Protein Science

Publish with us

Policies and ethics

Back to top

Search

Navigation