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  • © 2016

Proteostasis

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1449)

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Table of contents (31 protocols)

  1. Front Matter

    Pages i-xvi
  2. Review and Literature Mining on Proteostasis Factors and Cancer

    • Ana Sofia Carvalho, Manuel S. Rodríguez, Rune Matthiesen
    Pages 71-84
  3. Immunodepletion and Immunopurification as Approaches for CSN Research

    • Amnon Golan, Ning Wei, Elah Pick
    Pages 103-116
  4. Studying Protein Ubiquitylation in Yeast

    • Junie Hovsepian, Michel Becuwe, Oded Kleifeld, Michael H. Glickman, Sébastien Léon
    Pages 117-142
  5. Isolation of the Ubiquitin-Proteome from Tumor Cell Lines and Primary Cells Using TUBEs

    • Wendy Xolalpa, Lydia Mata-Cantero, Fabienne Aillet, Manuel S. Rodriguez
    Pages 161-175
  6. TUBEs-Mass Spectrometry for Identification and Analysis of the Ubiquitin-Proteome

    • Mikel Azkargorta, Iraide Escobes, Felix Elortza, Rune Matthiesen, Manuel S. Rodríguez
    Pages 177-192
  7. Isolation of Ubiquitinated Proteins to High Purity from In Vivo Samples

    • Juanma Ramirez, Mingwei Min, Rosa Barrio, Catherine Lindon, Ugo Mayor
    Pages 193-202
  8. Method for the Purification of Endogenous Unanchored Polyubiquitin Chains

    • Daniel Scott, Jo Strachan, Varun Gopala Krishna, Barry Shaw, David J. Tooth, Mark S. Searle et al.
    Pages 203-213
  9. Fluorescent Tools for In Vivo Studies on the Ubiquitin-Proteasome System

    • Olli Matilainen, Sweta Jha, Carina I. Holmberg
    Pages 215-222
  10. Monitoring Ubiquitin-Coated Bacteria via Confocal Microscopy

    • Marie Lork, Mieke Delvaeye, Amanda Gonçalves, Evelien Van Hamme, Rudi Beyaert
    Pages 243-250
  11. Detection and Analysis of Cell Cycle-Associated APC/C-Mediated Cellular Ubiquitylation In Vitro and In Vivo

    • Cesyen Cedeño, Esther La Monaca, Mara Esposito, Gustavo J. Gutierrez
    Pages 251-265
  12. Detection and Analysis of SUMOylation Substrates In Vitro and In Vivo

    • Cesyen Cedeño, Esther La Monaca, Mara Esposito, Gustavo J. Gutierrez
    Pages 267-278
  13. Dissecting SUMO Dynamics by Mass Spectrometry

    • Krzysztof Drabikowski, MichaÅ‚ Dadlez
    Pages 291-298
  14. Isolation of Lysosomes from Mammalian Tissues and Cultured Cells

    • Carmen Aguado, Eva Pérez-Jiménez, Marcos Lahuerta, Erwin Knecht
    Pages 299-311

About this book

This volume highlights the role of proteostasis in human health and associated disease model systems, reflecting its rising importance which has led to the development of new technologies to obtain insight into underling protein mechanistic events. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Proteostasis: Methods and Protocols aims to become a reference book on proteostasis in human health.



Editors and Affiliations

  • Computational and Experimental Biology Group Department of Health Promotion and Chronic Diseases, National Health Institute Dr. Ricardo Jorge, INSA, I.P., Lisboa, Portugal

    Rune Matthiesen

Bibliographic Information

  • Book Title: Proteostasis

  • Book Subtitle: Methods and Protocols

  • Editors: Rune Matthiesen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3756-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3754-7Published: 10 September 2016

  • Softcover ISBN: 978-1-4939-8130-4Published: 09 June 2018

  • eBook ISBN: 978-1-4939-3756-1Published: 09 September 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 488

  • Number of Illustrations: 34 b/w illustrations, 60 illustrations in colour

  • Topics: Protein Science

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access