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Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools

  • Book
  • © 2016

Overview

Editors:
  1. Maurizio Federico

    1. Istituto Superiore di Sanità, National AIDS Center, Rome, Italy

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1448)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
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  2. Recent Improvements in LV Construction, Production, and Transduction

    1. Front Matter

      Pages 1-1
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    2. Construction of Modular Lentiviral Vectors for Effective Gene Expression and Knockdown

      • Angeline de Bruyns, Ben Geiling, David Dankort
      Pages 3-21

    3. Development of Inducible Molecular Switches Based on All-in-One Lentiviral Vectors Equipped with Drug Controlled FLP Recombinase

      • Tobias Maetzig, Axel Schambach
      Pages 23-39

    4. Production of Retrovirus-Based Vectors in Mildly Acidic pH Conditions

      • Nathalie Holic, David Fenard
      Pages 41-48

    5. Optimized Lentiviral Transduction Protocols by Use of a Poloxamer Enhancer, Spinoculation, and scFv-Antibody Fusions to VSV-G

      • NataÅ¡a Anastasov, Ines Höfig, Sabine Mall, Angela M. Krackhardt, Christian Thirion
      Pages 49-61

  3. New LV Targets and Applications

    1. Front Matter

      Pages 63-63
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    2. Transduction of Murine Hematopoietic Stem Cells with Tetracycline-regulated Lentiviral Vectors

      • Maike Stahlhut, Axel Schambach, Olga S. Kustikova
      Pages 65-76

    3. Introduction of shRNAs, miRNAs, or AntagomiRs into Primary Human Liver Cells Through Lentiviral Vectors

      • Jessica K. Rieger, Maria Thomas
      Pages 77-84

    4. Production and Concentration of Lentivirus for Transduction of Primary Human T Cells

      • Alan Kennedy, Adam P. Cribbs
      Pages 85-93

    5. Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa Followed by In Vitro Fertilization and Embryo Transfer

      • Anil Chandrashekran, Colin Casimir, Nick Dibb, Carol Readhead, Robert Winston
      Pages 95-106

    6. The LAM-PCR Method to Sequence LV Integration Sites

      • Wei Wang, Cynthia C. Bartholomae, Richard Gabriel, Annette Deichmann, Manfred Schmidt
      Pages 107-120

    7. Conditional RNAi Using the Lentiviral GLTR System

      • Elisabeth Pfeiffenberger, Reinhard Sigl, Stephan Geley
      Pages 121-138

    8. Lentiviral Vectors for the Engineering of Implantable Cells Secreting Recombinant Antibodies

      • Aurélien Lathuilière, Bernard L. Schneider
      Pages 139-155

  4. Integrase-Mutant Lvs

    1. Front Matter

      Pages 157-157
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    2. Transient Expression of Green Fluorescent Protein in Integrase-Defective Lentiviral Vector-Transduced 293T Cell Line

      • Fazlina Nordin, Zariyantey Abdul Hamid, Lucas Chan, Farzin Farzaneh, M. K. Azaham A. Hamid
      Pages 159-173

    3. Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson’s Disease

      • Ngoc B. Lu-Nguyen, Martin Broadstock, Rafael J. Yáñez-Muñoz
      Pages 175-184

    4. Development of Lentiviral Vectors for Targeted Integration and Protein Delivery

      • Diana Schenkwein, Seppo Ylä-Herttuala
      Pages 185-198

  5. Production, Detection, and Engineering of Exosomes

    1. Front Matter

      Pages 199-199
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    2. Biogenesis and Functions of Exosomes and Extracellular Vesicles

      • Florian Dreyer, Andreas Baur
      Pages 201-216
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Keywords

About this book

This volume provides current methods and protocols for gene and protein delivery based on both lentivirus-generated and spontaneously released nanovesicles.  Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools guides readers through methods on macromolecule delivery and chapters describe the LV-based protocols of gene engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools aims to ensure successful results in the further study of this vital field.


Editors and Affiliations

  • Istituto Superiore di Sanità, National AIDS Center, Rome, Italy

    Maurizio Federico

Bibliographic Information

  • Book Title: Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools

  • Editors: Maurizio Federico

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3753-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3751-6Published: 18 June 2016

  • Softcover ISBN: 978-1-4939-8129-8Published: 31 May 2018

  • eBook ISBN: 978-1-4939-3753-0Published: 17 June 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 276

  • Number of Illustrations: 14 b/w illustrations, 33 illustrations in colour

  • Topics: Human Genetics

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