Protein Tyrosine Phosphatases

1. Front Matter

   Pages i-xii

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2. The Extended Family of Protein Tyrosine Phosphatases

   • Andrés Alonso, Caroline E. Nunes-Xavier, Yolanda Bayón, Rafael Pulido

   Pages 1-23

3. Global RT-PCR and RT-qPCR Analysis of the mRNA Expression of the Human PTPome

   • Caroline E. Nunes-Xavier, Rafael Pulido

   Pages 25-37

4. Expression, Purification, and Kinetic Analysis of PTP Domains

   • Mihaela Mentel, Rodica A. Badea, Georgiana Necula - Petrareanu, Sujay T. Mallikarjuna, Aura E. Ionescu, Stefan E. Szedlacsek

   Pages 39-66

5. Peptide Microarrays for Real-Time Kinetic Profiling of Tyrosine Phosphatase Activity of Recombinant Phosphatases and Phosphatases in Lysates of Cells or Tissue Samples

   • Liesbeth Hovestad-Bijl, Jeroen van Ameijde, Dirk Pijnenburg, Riet Hilhorst, Rob Liskamp, Rob Ruijtenbeek

   Pages 67-78

6. Tailor-Made Protein Tyrosine Phosphatases: In Vitro Site-Directed Mutagenesis of PTEN and PTPRZ-B

   • Sandra Luna, Janire Mingo, Olaia Aurtenetxe, Lorena Blanco, Laura Amo, Jan Schepens et al.

   Pages 79-93

7. Assays to Measure PTEN Lipid Phosphatase Activity In Vitro from Purified Enzyme or Immunoprecipitates

   • Laura Spinelli, Nicholas R. Leslie

   Pages 95-105

8. Assessing the Biological Activity of the Glucan Phosphatase Laforin

   • Carlos Romá-Mateo, Madushi Raththagala, Mathew S. Gentry, Pascual Sanz

   Pages 107-119

9. Discovery and Evaluation of PRL Trimer Disruptors for Novel Anticancer Agents

   • Yunpeng Bai, Zhi-Hong Yu, Zhong-Yin Zhang

   Pages 121-138

10. Analyzing Pseudophosphatase Function

    • Shantá D. Hinton

    Pages 139-153

11. Crystallization of PTP Domains

    • Colin Levy, James Adams, Lydia Tabernero

    Pages 155-180

12. NMR Spectroscopy to Study MAP Kinase Binding to MAP Kinase Phosphatases

    • Wolfgang Peti, Rebecca Page

    Pages 181-196

13. Visualizing and Quantitating the Spatiotemporal Regulation of Ras/ERK Signaling by Dual-Specificity Mitogen-Activated Protein Phosphatases (MKPs)

    • Christopher J. Caunt, Andrew M. Kidger, Stephen M. Keyse

    Pages 197-215

14. In Situ Proximity Ligation Assay (In Situ PLA) to Assess PTP-Protein Interactions

    • Sina Koch, Irene Helbing, Sylvia-Annette Böhmer, Makoto Hayashi, Lena Claesson-Welsh, Ola Söderberg et al.

    Pages 217-242

15. Use of Dominant-Negative/Substrate Trapping PTP Mutations to Search for PTP Interactors/Substrates

    • Vegesna Radha

    Pages 243-265

16. Detection and Identification of Ligands for Mammalian RPTP Extracellular Domains

    • Andrew William Stoker

    Pages 267-281

17. Production of Osteoclasts for Studying Protein Tyrosine Phosphatase Signaling

    • Eynat Finkelshtein, Einat Levy-Apter, Ari Elson

    Pages 283-300

18. Functional Analysis of Protein Tyrosine Phosphatases in Thrombosis and Hemostasis

    • Souad Rahmouni, Alexandre Hego, Céline Delierneux, Odile Wéra, Lucia Musumeci, Lutz Tautz et al.

    Pages 301-330

19. Functional Analysis of Dual-Specificity Protein Phosphatases in Angiogenesis

    • Mathieu Amand, Charlotte Erpicum, Christine Gilles, Agnès Noël, Souad Rahmouni

    Pages 331-349

20. Studying Protein-Tyrosine Phosphatases in Zebrafish

    • Alexander James Hale, Jeroen den Hertog

    Pages 351-372

This book provides coverage, methodology, and laboratory protocols on the more essential aspects of protein tyrosine phosphatase (PTP) function and regulation, including the use of standardized in vitro functional assays, suitable cell systems, and animal and microorganism models. Chapters covering state-of-the-art technical approaches suitable to decipher the physiologic roles of PTPs, and their involvement in tissue-specific functions, are also included, which will be of utility for both newcomers and experienced researchers in the field of tyrosine- and phosphoinositide- phosphorylation/dephosphorylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Tyrosine Phosphatases: Methodsand Protocols aims to aid researchers in better defining the common and individual features of the PTP family members and translating this knowledge into PTP-based therapy for human disease.

• Cell signaling proteins
• PTP function and regulation
• Phosphorylation
• Dephosphorylation
• Protein kinases
• PTP family members
• Therapy for human disease

• BioCruces Health Research Institute, Barakaldo, Bizkaia, Spain

  Rafael Pulido

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