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Human Respiratory Syncytial Virus

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Ralph A. Tripp

    1. College of Veterinary Medicine, University of Georgia, Athens, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Patricia A. Jorquera

    1. Department of Infectious Diseases, University of Georgia College of Veterinary Medicine, Athens, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1442)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Human Respiratory Syncytial Virus: An Introduction

    • Patricia A. Jorquera, Lydia Anderson, Ralph A. Tripp
    Pages 1-12

  3. RSV Growth and Quantification by Microtitration and qRT-PCR Assays

    • Hayat Caidi, Jennifer L. Harcourt, Lia M. Haynes
    Pages 13-32

  4. Quantification of RSV Infectious Particles by Plaque Assay and Immunostaining Assay

    • Patricia A. Jorquera, Ralph A. Tripp
    Pages 33-40

  5. Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays

    • Samadhan J. Jadhao, Larry J. Anderson
    Pages 41-52

  6. Secretory Expression and Purification of Respiratory Syncytial Virus G and F Proteins in Human Cells

    • Samadhan J. Jadhao, Larry J. Anderson
    Pages 53-62

  7. Development of Human Monoclonal Antibodies Against Respiratory Syncytial Virus Using a High Efficiency Human Hybridoma Technique

    • Gabriela Alvarado, James E. Crowe Jr.
    Pages 63-76

  8. Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune Protection

    • Pedro A. Piedra, Anne M. Hause, Letisha Aideyan
    Pages 77-91

  9. Host Factors Modulating RSV Infection: Use of Small Interfering RNAs to Probe Functional Importance

    • Leon Caly, Hong-mei Li, David Jans
    Pages 93-117

  10. In Vitro Modeling of RSV Infection and Cytopathogenesis in Well-Differentiated Human Primary Airway Epithelial Cells (WD-PAECs)

    • Lindsay Broadbent, Remi Villenave, Hong Guo-Parke, Isobel Douglas, Michael D. Shields, Ultan F. Power
    Pages 119-139

  11. Reverse Genetics of Respiratory Syncytial Virus

    • Christopher C. Stobart, Anne L. Hotard, Jia Meng, Martin L. Moore
    Pages 141-153

  12. Use of Minigenome Systems to Study RSV Transcription

    • Michael N. Teng, Kim C. Tran
    Pages 155-164

  13. Screening for Host Factors Directly Interacting with RSV Protein: Microfluidics

    • Sarit Kipper, Dorit Avrahami, Monika Bajorek, Doron Gerber
    Pages 165-174

  14. A Proteomic-Based Workflow Using Purified Respiratory Syncytial Virus Particles to Identify Cellular Factors as Drug Targets

    • Tra Nguyen Huong, Boon Huan Tan, Richard J. Sugrue
    Pages 175-194

  15. MicroRNA Profiling from RSV-Infected Biofluids, Whole Blood, and Tissue Samples

    • Lydia Anderson, Patricia A. Jorquera, Ralph A. Tripp
    Pages 195-208

  16. Mouse and Cotton Rat Models of Human Respiratory Syncytial Virus

    • Penny A. Rudd, Weiqiang Chen, Suresh Mahalingam
    Pages 209-217

  17. In Vivo Assessment of Airway Function in the Mouse Model

    • Azzeddine Dakhama, Erwin W. Gelfand
    Pages 219-230

  18. Evaluation of the Adaptive Immune Response to Respiratory Syncytial Virus

    • Cory J. Knudson, Kayla A. Weiss, Megan E. Stoley, Steven M. Varga
    Pages 231-243

  19. Back Matter

    Pages 245-247
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Keywords

About this book

This volume summarizes current techniques; ranging from protocols for virus growth, isolation, quantification and generation of recombinant RSV virus to procedures for the efficient characterization of the host immune response to RSV infection. These techniques are used in numerous laboratories around the world and are the building blocks that support the majority of RSV virus research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Humana Respiratory Syncytial Virus: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • College of Veterinary Medicine, University of Georgia, Athens, USA

    Ralph A. Tripp

  • Department of Infectious Diseases, University of Georgia College of Veterinary Medicine, Athens, USA

    Patricia A. Jorquera

Bibliographic Information

  • Book Title: Human Respiratory Syncytial Virus

  • Book Subtitle: Methods and Protocols

  • Editors: Ralph A. Tripp, Patricia A. Jorquera

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3687-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3685-4Published: 27 July 2016

  • Softcover ISBN: 978-1-4939-8117-5Published: 20 June 2018

  • eBook ISBN: 978-1-4939-3687-8Published: 27 July 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 247

  • Number of Illustrations: 16 b/w illustrations, 23 illustrations in colour

  • Topics: Infectious Diseases

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