Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1439)
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Table of contents (18 protocols)
Keywords
About this book
This third edition volume
expands on the previous editions both by presenting more detailed protocols for
the techniques described in the first and second editions of High Throughput
Screening: Methods and Protocols
and by covering important new procedures. The first chapter of
this book provides an overview of important assay development techniques, while
the rest of the chapters detail how to develop and execute screens at whatever
throughput the user needs. Some chapter examples are: structure-based virtual
screening, high throughput screening using mass spectrometry, identification of state-dependent
blockers for voltage gated calcium channels, bioluminescence resonance
energy transfer platform to monitor protein-protein interactions in live cells,
high throughput flow cytometry, and application of imaging-based assays in microplate formats for high
content screening. Written in the highly successful Methods in Molecular
Biology series format, chaptersinclude introductions to their respective
topics, lists of the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and avoiding
known pitfalls.
Cutting edge and thorough, High Throughput Screening: Methods and Protocols,
Third Edition, is a valuable resource for anyone who is interested in HTS
research.
Editors and Affiliations
Bibliographic Information
Book Title: High Throughput Screening
Book Subtitle: Methods and Protocols
Editors: William P. Janzen
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3673-1
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3671-7Published: 18 June 2016
Softcover ISBN: 978-1-4939-8113-7Published: 31 May 2018
eBook ISBN: 978-1-4939-3673-1Published: 17 June 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 3
Number of Pages: XI, 308
Number of Illustrations: 30 b/w illustrations, 71 illustrations in colour
Topics: Biotechnology