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  • © 2016

West Nile Virus

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1435)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
  2. A Brief Review of West Nile Virus Biology

    • Berlin Londono-Renteria, Tonya M. Colpitts
    Pages 1-13
  3. Overview of West Nile Virus Transmission and Epidemiology

    • Andrea Troupin, Tonya M. Colpitts
    Pages 15-18
  4. Propagation and Titration of West Nile Virus on Vero Cells

    • Alexander J. McAuley, David W. C. Beasley
    Pages 19-27
  5. Generating West Nile Virus from an Infectious Clone

    • Rianna Vandergaast, Brenda L. Fredericksen
    Pages 29-43
  6. Functional Analysis of West Nile Virus Proteins in Human Cells

    • Pakieli H. Kaufusi, Alanna Tseng, Vivek R. Nerurkar
    Pages 45-60
  7. Lessons from the Murine Models of West Nile Virus Infection

    • Brenna McGruder, Vandana Saxena, Tian Wang
    Pages 61-69
  8. ELISA and Neutralization Methods to Measure Anti-West Nile Virus Antibody Responses

    • Brian M. Friedrich, David W. C. Beasley
    Pages 129-141
  9. Safe Handling of West Nile Virus in the Insectary

    • Dexter R. McKellar, Michael J. Conway
    Pages 143-150
  10. Field Surveillance Methods for West Nile Virus

    • Samuel B. Jameson, Dawn M. Wesson
    Pages 165-174
  11. Detection Protocols for West Nile Virus in Mosquitoes, Birds, and Nonhuman Mammals

    • Elizabeth B. Kauffman, Mary A. Franke, Laura D. Kramer
    Pages 175-206
  12. Field Methods and Sample Collection Techniques for the Surveillance of West Nile Virus in Avian Hosts

    • Sarah S. Wheeler, Walter M. Boyce, William K. Reisen
    Pages 207-220
  13. Role of Immune Aging in Susceptibility to West Nile Virus

    • Yi Yao, Ruth R. Montgomery
    Pages 235-247

About this book

This volume is a collection of research methods, techniques, and approaches to investigate the molecular biology of West Nile Virus (WNV). Chapters in the book cover many facets of WNV, such as propagation and titration of WNV in vero cells, examination of WNV neuroinvasion and neuropathogenesis in the central nervous system of a murine model, field surveillance methods for WNV, and in vitro and in vivo blood-brain barrier models to study WNV pathogenesis. A brief introduction, along with a discussion on WNV laboratory safety are also included. Written in the highly successful Methods in Molecular Biology series format, chapters includes introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, West Nile Virus will interest virologists, molecular biologists, microbiologists, and other scientists working with WNV. This book is a comprehensive guide for those conducting research in the field of WNV biology, including life cycles, pathogenesis, impact of infection, and possible treatment/prevention development.

Editors and Affiliations

  • Dept. of Path., MicroBio., and Immun., Univ. of South Carolina School of Med., Columbia, USA

    Tonya M. Colpitts

Bibliographic Information

  • Book Title: West Nile Virus

  • Book Subtitle: Methods and Protocols

  • Editors: Tonya M. Colpitts

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3670-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3668-7Published: 18 May 2016

  • Softcover ISBN: 978-1-4939-8112-0Published: 26 May 2018

  • eBook ISBN: 978-1-4939-3670-0Published: 17 May 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 295

  • Number of Illustrations: 7 b/w illustrations, 25 illustrations in colour

  • Topics: Infectious Diseases

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access