Skip to main content
SpringerLink
Log in

Gap Junction Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Mathieu Vinken

    1. Department of in Vitro Toxicology and D, Vrije Universiteit Brussel, Brussels, Belgium

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Scott R. Johnstone

    1. BHF Glasgow Cardiovascular Research, University of Glasgow, Glasgow, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols involving gap junctions
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1437)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (16 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction

    • Michaël Maes, Joost Willebrords, Sara Crespo Yanguas, Bruno Cogliati, Mathieu Vinken
    Pages 1-19

  3. DNA Methylation Analysis of Human Tissue-Specific Connexin Genes

    • Xiaoming Liu, Canxia Xu
    Pages 21-36

  4. Detection of Connexins in Liver Cells Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis

    • Joost Willebrords, Michaël Maes, Sara Crespo Yanguas, Bruno Cogliati, Mathieu Vinken
    Pages 37-53

  5. Immunohisto- and Cytochemistry Analysis of Connexins

    • Bruno Cogliati, Michaël Maes, Isabel Veloso Alves Pereira, Joost Willebrords, Tereza Cristina Da Silva, Sara Crespo Yanguas et al.
    Pages 55-70

  6. Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells

    • Miranda E. Good, Daniela Begandt, Leon J. DeLalio, Scott R. Johnstone, Brant E. Isakson
    Pages 71-82

  7. Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy and Labor

    • Mark Kibschull, Stephen J. Lye, Oksana Shynlova
    Pages 83-95

  8. Identification of Connexin43 Phosphorylation and S-Nitrosylation in Cultured Primary Vascular Cells

    • Alexander W. Lohman, Adam C. Straub, Scott R. Johnstone
    Pages 97-111

  9. Preparation of Gap Junctions in Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome Analysis

    • Stephanie Fowler, Mark Akins, Steffany A. L. Bennett
    Pages 113-132

  10. Scrape Loading/Dye Transfer Assay

    • Pavel Babica, Iva Sovadinová, Brad L. Upham
    Pages 133-144

  11. Microinjection Technique for Assessment of Gap Junction Function

    • Michael D. Fridman, Jun Liu, Yu Sun, Robert M. Hamilton
    Pages 145-154

  12. Electroporation Loading and Dye Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling

    • Elke Decrock, Marijke De Bock, Diego De Baere, Delphine Hoorelbeke, Nan Wang, Luc Leybaert
    Pages 155-169

  13. Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro

    • Maria Kuzma-Kuzniarska, Clarence Yapp, Philippa A. Hulley
    Pages 171-179

  14. Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photoactivation and Fluorescence Imaging

    • Song Yang, Wen-Hong Li
    Pages 181-191

  15. A Functional Assay to Assess Connexin 43-Mediated Cell-to-Cell Communication of Second Messengers in Cultured Bone Cells

    • Joseph P. Stains, Roberto Civitelli
    Pages 193-201

  16. Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junctions and Hemichannels

    • Jegan Iyyathurai, Bernard Himpens, Geert Bultynck, Catheleyne D’hondt
    Pages 203-211

  17. Establishment of the Dual Whole Cell Recording Patch Clamp Configuration for the Measurement of Gap Junction Conductance

    • Richard D. Veenstra
    Pages 213-231

  18. Back Matter

    Pages 233-235
    Download chapter PDF
Back to top

Keywords

About this book

Presenting state-of-the-art protocols to study gap junctions, this detailed book first focuses on the use of methods and tools to investigate the different aspects of connexin expression and gap junction regulation. The second part of the volume describes several methods to probe gap junction functionality as such. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Gap Junction Protocols is intended for basic and applied researchers in the area of biomedical and life sciences, both in academic and industrial settings.

Editors and Affiliations

  • Department of in Vitro Toxicology and D, Vrije Universiteit Brussel, Brussels, Belgium

    Mathieu Vinken

  • BHF Glasgow Cardiovascular Research, University of Glasgow, Glasgow, United Kingdom

    Scott R. Johnstone

Bibliographic Information

  • Book Title: Gap Junction Protocols

  • Editors: Mathieu Vinken, Scott R. Johnstone

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3664-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2016

  • Hardcover ISBN: 978-1-4939-3662-5Published: 21 May 2016

  • Softcover ISBN: 978-1-4939-8110-6Published: 26 May 2018

  • eBook ISBN: 978-1-4939-3664-9Published: 20 May 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 235

  • Number of Illustrations: 11 b/w illustrations, 34 illustrations in colour

  • Topics: Cell Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation