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Chromosome Architecture

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Mark C. Leake

    1. Biological Physical Sciences Instit, University of York, York, United Kingdom

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1431)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. New Advances in Chromosome Architecture

    • Mark C. Leake
    Pages 1-3

  3. Single-Molecule Narrow-Field Microscopy of Protein–DNA Binding Dynamics in Glucose Signal Transduction of Live Yeast Cells

    • Adam J. M. Wollman, Mark C. Leake
    Pages 5-15

  4. Single-Molecule Imaging to Characterize the Transport Mechanism of the Nuclear Pore Complex

    • Grace Jeremy, James Stevens, Alan R. Lowe
    Pages 17-35

  5. Using Fluorescence Recovery After Photobleaching (FRAP) to Study Dynamics of the Structural Maintenance of Chromosome (SMC) Complex In Vivo

    • Anjana Badrinarayanan, Mark C. Leake
    Pages 37-46

  6. Imaging DNA Structure by Atomic Force Microscopy

    • Alice L. B. Pyne, Bart W. Hoogenboom
    Pages 47-60

  7. Investigating Bacterial Chromosome Architecture

    • Christian Lesterlin, Nelly Duabrry
    Pages 61-72

  8. Transverse Magnetic Tweezers Allowing Coincident Epifluorescence Microscopy on Horizontally Extended DNA

    • Stephen J. Cross, Claire E. Brown, Christoph G. Baumann
    Pages 73-90

  9. Probing Chromosome Dynamics in Bacillus subtilis

    • Alan Koh, Heath Murray
    Pages 91-108

  10. Systems Biology Approaches for Understanding Genome Architecture

    • Sven Sewitz, Karen Lipkow
    Pages 109-126

  11. In Vivo and In Situ Replication Labeling Methods for Super-resolution Structured Illumination Microscopy of Chromosome Territories and Chromatin Domains

    • Ezequiel Miron, Cassandravictoria Innocent, Sophia Heyde, Lothar Schermelleh
    Pages 127-140

  12. DNA–Protein Interactions Studied Directly Using Single Molecule Fluorescence Imaging of Quantum Dot Tagged Proteins Moving on DNA Tightropes

    • Luke Springall, Alessio V. Inchingolo, Neil M. Kad
    Pages 141-150

  13. Escherichia coli Chromosome Copy Number Measurement Using Flow Cytometry Analysis

    • Michelle Hawkins, John Atkinson, Peter McGlynn
    Pages 151-159

  14. Bacterial Chromosome Dynamics by Locus Tracking in Fluorescence Microscopy

    • Avelino Javer, Marco Cosentino Lagomarsino, Pietro Cicuta
    Pages 161-173

  15. Biophysical Characterization of Chromatin Remodeling Protein CHD4

    • Rosa Morra, Tomas Fessl, Yuchong Wang, Erika J. Mancini, Roman Tuma
    Pages 175-193

  16. Atomistic Molecular Dynamics Simulations of DNA Minicircle Topoisomers: A Practical Guide to Setup, Performance, and Analysis

    • Thana Sutthibutpong, Agnes Noy, Sarah Harris
    Pages 195-219

  17. Super-Resolution Microscopy and Tracking of DNA-Binding Proteins in Bacterial Cells

    • Stephan Uphoff
    Pages 221-234

  18. Studying the Dynamics of Chromatin-Binding Proteins in Mammalian Cells Using Single-Molecule Localisation Microscopy

    • Srinjan Basu, Yi Lei Tan, Edward J. R. Taylor, Ernest D. Laue, Steven F. Lee
    Pages 235-263

  19. Intra-Nuclear Single-Particle Tracking (I-SPT) to Reveal the Functional Architecture of Chromosomes

    • Vincent Récamier
    Pages 265-274

  20. Visualizing Bacillus subtilis During Vegetative Growth and Spore Formation

    • Xindan Wang, Paula Montero Llopis
    Pages 275-287
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Keywords

About this book

This volume details a valuable collection of protocols and reviews, such as emerging experimental and theoretical approaches. These approaches have resulted in a substantial improvement in the understanding of chromosome architecture. Chromosome Architecture: Methods and Protocols guides readers through cutting-edge interdisciplinary methods which allow for an understanding of architecture of chromosomes with exceptionally enhanced resolution, both in terms of space and time. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Chromosome Architecture: Methods and Protocols aims to ensure successful results in the further study of this vital field.


Editors and Affiliations

  • Biological Physical Sciences Instit, University of York, York, United Kingdom

    Mark C. Leake

Bibliographic Information

  • Book Title: Chromosome Architecture

  • Book Subtitle: Methods and Protocols

  • Editors: Mark C. Leake

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3631-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3629-8Published: 10 June 2016

  • Softcover ISBN: 978-1-4939-8100-7Published: 26 May 2018

  • eBook ISBN: 978-1-4939-3631-1Published: 09 June 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 290

  • Number of Illustrations: 12 b/w illustrations, 65 illustrations in colour

  • Topics: Human Genetics

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