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Plant Cytogenetics

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Shahryar F. Kianian

    1. University of Minnesota, St. Paul, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Penny M. Avoles Kianian

    1. University of Minnesota, St. Paul, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1429)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. C-Banding of Plant Chromosomes

    • Eric N. Jellen
    Pages 1-5

  3. Chromosome Painting by GISH and Multicolor FISH

    • Steven S. Xu, Zhao Liu, Qijun Zhang, Zhixia Niu, Chao-Chien Jan, Xiwen Cai
    Pages 7-21

  4. Fluorescent In Situ Hybridization on Extended Chromatin Fibers for High-Resolution Analysis of Plant Chromosomes

    • Daryna Dechyeva, Thomas Schmidt
    Pages 23-33

  5. Tyramide Signal Amplification: Fluorescence In Situ Hybridization for Identifying Homoeologous Chromosomes

    • Araceli Fominaya, Yolanda Loarce, Juan M. González, Esther Ferrer
    Pages 35-48

  6. Localization of Low-Copy DNA Sequences on Mitotic Chromosomes by FISH

    • Miroslava Karafiátová, Jan BartoÅ¡, Jaroslav Doležel
    Pages 49-64

  7. Immunolabeling and In Situ Labeling of Isolated Plant Interphase Nuclei

    • Ali Pendle, Peter Shaw
    Pages 65-76

  8. Manipulation of Homologous and Homoeologous Chromosome Recombination in Wheat

    • Adam J. Lukaszewski
    Pages 77-89

  9. Dissecting Plant Chromosomes by the Use of Ionizing Radiation

    • Penny M. A. Kianian, Katie L. Liberatore, Marisa E. Miller, Justin B. Hegstad, Shahryar F. Kianian
    Pages 91-101

  10. Optical Nano-mapping and Analysis of Plant Genomes

    • Ming-Cheng Luo, Karin R. Deal, Armond Murray, Tingting Zhu, Alex R. Hastie, Will Stedman et al.
    Pages 103-117

  11. Flow Sorting Plant Chromosomes

    • Jan Vrána, Petr Cápal, Jarmila Číhalíková, Marie Kubaláková, Jaroslav Doležel
    Pages 119-134

  12. Construction of BAC Libraries from Flow-Sorted Chromosomes

    • Jan Å afář, Hana Å imková, Jaroslav Doležel
    Pages 135-149

  13. The Chromosome Microdissection and Microcloning Technique

    • Ying-Xin Zhang, Chuan-Liang Deng, Zan-Min Hu
    Pages 151-160

  14. Immunolocalization on Whole Anther Chromosome Spreads for Male Meiosis

    • Stefanie Dukowic-Schulze, Anthony Harris, Changbin Chen
    Pages 161-175

  15. Mapping Recombination Initiation Sites Using Chromatin Immunoprecipitation

    • Yan He, Minghui Wang, Qi Sun, Wojciech P. Pawlowski
    Pages 177-188

  16. Chromatin Immunoprecipitation to Study The Plant Epigenome

    • Zidian Xie, Gernot Presting
    Pages 189-196

  17. Back Matter

    Pages 197-198
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Keywords

About this book

This volume covers a range of methods used in plant cytogenetics, beginning with basic analysis of chromosomes and visualizing gene locations, to manipulating and dissecting chromosomes, and then focusing on less understood features of chromosomes such as recombination initiation sites and epigenomic marks. The methods described in Plant Cytogenetics: Methods and Protocols build on each other and provide, those new to the field, with a comprehensive platform to support their research endeavours, while also introducing advanced techniques to experienced researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, Plant Cytogenetics: Methods and Protocols, is a valuable resource for anyone who is interested in the diverse and wonderfully complex field of cytogenetics. 

Editors and Affiliations

  • University of Minnesota, St. Paul, USA

    Shahryar F. Kianian, Penny M. Avoles Kianian

About the editors

Shahryar Kianian Research Leader USDA-ARS Cereal Disease Laboratory University of Minnesota St. Paul, MN 55108 
Email: Shahryar.Kianian@ars.usda.gov

Penny M Avoles Kianian Alderman Hall, St. Paul, MN 55108  
Email:P.Kianian@gmail.com 

Bibliographic Information

  • Book Title: Plant Cytogenetics

  • Book Subtitle: Methods and Protocols

  • Editors: Shahryar F. Kianian, Penny M. Avoles Kianian

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3622-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3620-5Published: 10 August 2016

  • Softcover ISBN: 978-1-4939-8097-0Published: 09 June 2018

  • eBook ISBN: 978-1-4939-3622-9Published: 10 August 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 198

  • Number of Illustrations: 8 b/w illustrations, 32 illustrations in colour

  • Topics: Plant Sciences

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