Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1423)
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Table of contents (22 protocols)
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Front Matter
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Introduction
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Front Matter
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In Vitro Culture of Dendritic Cells
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Front Matter
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Purification and Isolation of Dendritic Cell Subsets
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Front Matter
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Ex Vivo Functional Analysis of Dendritic Cells
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Front Matter
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Keywords
About this book
The third edition of this volume is aimed at providing both beginners and more experienced researchers a choice of methods to isolate and analyze dendritic cells(DC). An introductory review provides an overview of recent advances in the characterization of DC subsets in mouse and human. While additional chapters provide methods to culture human and mouse dendritic cells, protocols for the isolation of dendritic cells, the isolation of dendritic cell progenitors from mouse, and the purification of dendritic cells from human blood. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Dendritic Cell Protocols, Third Edition aims to ensure successful results in the further study of this vital field.
Editors and Affiliations
Bibliographic Information
Book Title: Dendritic Cell Protocols
Editors: Elodie Segura, Nobuyuki Onai
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3606-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3604-5Published: 04 May 2016
Softcover ISBN: 978-1-4939-8092-5Published: 26 May 2018
eBook ISBN: 978-1-4939-3606-9Published: 03 May 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 3
Number of Pages: XI, 322
Number of Illustrations: 41 b/w illustrations, 27 illustrations in colour
Topics: Immunology