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  • © 2016

Gastrointestinal Physiology and Diseases

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1422)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xv
  2. In Vitro and Ex Vivo Systems to Study Gastrointestinal Functions and Diseases

    1. Front Matter

      Pages 1-1
    2. Lentivirus-Based Stable Gene Delivery into Intestinal Organoids

      • Yoshiaki Maru, Kaoru Orihashi, Yoshitaka Hippo
      Pages 13-21
    3. Co-culture of Gastric Organoids and Immortalized Stomach Mesenchymal Cells

      • Nina Bertaux-Skeirik, Jomaris Centeno, Rui Feng, Michael A. Schumacher, Ramesh A. Shivdasani, Yana Zavros
      Pages 23-31
    4. Ussing Chamber Technique to Measure Intestinal Epithelial Permeability

      • Sadasivan Vidyasagar, Gordon MacGregor
      Pages 49-61
    5. HPLC-Based Metabolomic Analysis of Normal and Inflamed Gut

      • Daniel J. Kao, Jordi M. Lanis, Erica Alexeev, Douglas J. Kominsky
      Pages 63-75
    6. NMR-Based Metabolomic Analysis of Normal and Inflamed Gut

      • Daniel J. Kao, Jordi M. Lanis, Erica Alexeev, Douglas J. Kominsky
      Pages 77-87
  3. Imaging Analysis of the Gastrointestinal System In Vivo

    1. Front Matter

      Pages 101-101
    2. Imaging Inflammatory Hypoxia in the Murine Gut

      • Alyssa K. Whitney, Eric L. Campbell
      Pages 115-126
    3. Label-Free Imaging of Eosinophilic Esophagitis Mouse Models Using Optical Coherence Tomography

      • Aneesh Alex, Elia D. Tait Wojno, David Artis, Chao Zhou
      Pages 127-136
    4. Near-Infrared Fluorescence Endoscopy to Detect Dysplastic Lesions in the Mouse Colon

      • Elias Gounaris, Yasushige Ishihara, Manisha Shrivastrav, David Bentrem, Terrence A. Barrett
      Pages 137-147
    5. Visualization of Signaling Molecules During Neutrophil Recruitment in Transgenic Mice Expressing FRET Biosensors

      • Rei Mizuno, Yuji Kamioka, Yoshiharu Sakai, Michiyuki Matsuda
      Pages 149-160
  4. Isolation and Characterization of Intestinal Immune Cells

    1. Front Matter

      Pages 169-169
    2. Macrophage Isolation from the Mouse Small and Large Intestine

      • Akihito Harusato, Duke Geem, Timothy L. Denning
      Pages 171-180

About this book

This volume provides a comprehensive collection of  classical and cutting edge protocols and techniques to examine the normal development and physiological functions of the gastrointestinal system and to model the most common digestive diseases. The chapters focus on diverse research topics including ex vivo systems to study gastrointestinal development and functions, in vivo imaging of the gastrointestinal tract, isolation and characterization of intestinal immune cells, and animal models of gastrointestinal inflammation and cancer. The Gastrointestinal Physiology and Diseases: Methods and Protocols book targets wide audience of physiologists, cell and developmental biologists, immunologists, and physician-scientists working in the field of gastroenterology and beyond. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Highly practical and clearly written, Gastrointestinal Physiology and Diseases: Methods and Protocols will serve both seasoned researchers as well as newcomers to the field and will provide a unique resource and expert guidance to modern laboratory techniques developed for examining normal functions and diseases of the gastrointestinal tract.


Editors and Affiliations

  • Dept of Human and Molecular Genetics, VCU Institute of Molecular Medicine, VCU, Richmond, USA

    Andrei I. Ivanov

Bibliographic Information

  • Book Title: Gastrointestinal Physiology and Diseases

  • Book Subtitle: Methods and Protocols

  • Editors: Andrei I. Ivanov

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3603-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3601-4Published: 01 June 2016

  • Softcover ISBN: 978-1-4939-8091-8Published: 26 May 2018

  • eBook ISBN: 978-1-4939-3603-8Published: 31 May 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 353

  • Number of Illustrations: 25 b/w illustrations, 62 illustrations in colour

  • Topics: Molecular Medicine

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access