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Forensic DNA Typing Protocols

  • Book
  • © 2016

Overview

Editors:
  1. William Goodwin

    1. School of Forensic and Applied Sciences, University of Central Lancashire, Preston, United Kingdom

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1420)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Collection of Samples for DNA Analysis

    • Roland A. H. van Oorschot, Timothy J. Verdon, Kaye N. Ballantyne
    Pages 1-12

  3. Body Fluid Identification Using mRNA Profiling

    • Amy D. Roeder, Cordula Haas
    Pages 13-31

  4. mRNA Profiling for Vaginal Fluid and Menstrual Blood Identification

    • Joanna Jakubowska, Agnieszka Maciejewska, Ryszard Pawłowski
    Pages 33-42

  5. Preservation of and DNA Extraction from Muscle Tissue

    • Dennis McNevin
    Pages 43-53

  6. DNA Extraction: Organic and Solid-Phase

    • Wafa Altayari
    Pages 55-68

  7. Extraction of DNA from Skeletal Remains

    • Suni M. Edson, Timothy P. McMahon
    Pages 69-87

  8. Extraction of DNA from Human Skeletal Material

    • Irena Zupanič Pajnič
    Pages 89-108

  9. The Development and Use of Internal Amplification Controls (IACs) with DNA Profiling Kits for Forensic DNA Analysis

    • Nathalie Zahra, William Goodwin
    Pages 109-124

  10. NucleoSpin® XS Columns for DNA Concentration and Clean-Up

    • William R. Hudlow
    Pages 125-129

  11. Purification of PCR Products to Improve STR Profiles

    • Amy D. Roeder
    Pages 131-134

  12. Analysis of 30 Biallelic INDEL Markers Using the Investigator DIPplex® Kit

    • Majid Bashir, Nur Haliza Bt Hassan
    Pages 135-142

  13. Analysis of Mitochondrial Control Region Using Sanger Sequencing

    • David Ballard
    Pages 143-155

  14. Whole Human Mitochondrial DNA Sequencing

    • Igor V. Ovchinnikov, Mathew J. Malek, Katelyn Kjelland, Kenneth Drees
    Pages 157-171

  15. In-Solution Hybridization for the Targeted Enrichment of the Whole Mitochondrial Genome

    • B. Bekaert, R. Ellerington, L. Van den Abbeele, R. Decorte
    Pages 173-183

  16. Enhanced DNA Profiling of the Semen Donor in Late Reported Sexual Assaults: Use of Y-Chromosome-Targeted Pre-amplification and Next Generation Y-STR Amplification Systems

    • Erin K. Hanson, Jack Ballantyne
    Pages 185-200

  17. Analysis of Rapidly Mutating Y Chromosome Short Tandem Repeats (RM Y-STRs)

    • Sibte Hadi
    Pages 201-211

  18. A Practical Guide to the HIrisPlex System: Simultaneous Prediction of Eye and Hair Color from DNA

    • Susan Walsh, Manfred Kayser
    Pages 213-231

  19. Inference of Ancestry in Forensic Analysis I: Autosomal Ancestry-Informative Marker Sets

    • Chris Phillips, Carla Santos, Manuel Fondevila, Ángel Carracedo, Maria Victoria Lareu
    Pages 233-253

  20. Inference of Ancestry in Forensic Analysis II: Analysis of Genetic Data

    • Carla Santos, Chris Phillips, A. Gomez-Tato, J. Alvarez-Dios, Ángel Carracedo, Maria Victoria Lareu
    Pages 255-285
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Keywords

About this book

This volume presents a series of protocols and methods, some of which are not widely used by researchers/practitioners, and will aid in the execution of different laboratory techniques. Forensic DNA Typing Protocols, Second Edition is arranged into a series of related chapters. Chapter 1-3 examines two different aspects of RNA analysis for body fluid identification. Chapters 4-7 focuses on the storage of biological materials and the extraction of DNA from hard tissues. Chapters 8-10 present methods for monitoring the quality of DNA extracts, and steps to aid in the purification of DNA. Chapters 11-16 talk about methods on non-standard markers, such as INDELs, Y chromosome STRs, and mitochondrial DNA. Detailed procedures and data analysis for phenotypes and ancestry are explored in Chapter 17-19. The last chapter (20) looks at the application of DNA typing to the identification of non-human material to species level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, Forensic DNA Typing Protocols, Second Edition, is a valuable resource for forensic specialists, researchers, and anyone interested in the field of forensic science.

 

Reviews

“This is an instructional book that raises awareness of the technology available in genetic forensic sciences. … The book is written in a way that is easy to understand for advanced professionals as well as beginners. Anyone wanting to understand the technology involved in forensic DNA analysis would benefit from this unique book. … The value of this book lies in the simple and logical way it presents complex information in forensic medicine.” (Luis F. Escobar, Doody's Book Reviews, November, 2016)

Editors and Affiliations

  • School of Forensic and Applied Sciences, University of Central Lancashire, Preston, United Kingdom

    William Goodwin

Bibliographic Information

  • Book Title: Forensic DNA Typing Protocols

  • Editors: William Goodwin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3597-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3595-6Published: 04 June 2016

  • Softcover ISBN: 978-1-4939-8089-5Published: 26 May 2018

  • eBook ISBN: 978-1-4939-3597-0Published: 03 June 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 298

  • Number of Illustrations: 10 b/w illustrations, 18 illustrations in colour

  • Topics: Human Genetics, Forensic Medicine

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