Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1419)
Buy it now
Buying options
Tax calculation will be finalised at checkout
Other ways to access
This is a preview of subscription content, log in via an institution to check for access.
Table of contents (20 protocols)
-
Front Matter
About this book
This volume contains
protocols specifically designed for studying programmed cell death, and also
discusses recent advances in techniques that span broader areas of biology that
have been recently used or that have potential to be incorporated into cell death
research. The protocols are mostly described in the context of mammalian
systems, but also cover other systems such as plants, Drosophila, and
yeast. Programmed Cell Death: Methods and Protocols is comprised of 20
chapters: Chapters 1-5 describe apoptosis detection techniques; Chapter 6-9
describe methods for studying apoptosis associated with various pathologies in
different organs including the lymphoid compartment, intestinal epithelium,
granulocytes, and cardiomyocytes; Chapter 11-13 cover protocols and techniques
for studying apoptosis in non-mammalian systems; Chapters 14-16 cover
biochemical and biophysical methods for studying Bcl-2 family protein dynamics
and protein-protein interactions during apoptosis;and the last four chapters
explore protocols that are useful not only in apoptosis research but in wider
areas of biological research, such as genome editing, inducible transgenes, and
proteomics. Written in the highly successful Methods in Molecular Biology
aeries format, chapters include introductions to their respective topics, lists
of the necessary material and reagents, step-by-step, readily reproducible
laboratory protocol, and tips on troubleshooting and avoiding known pitfalls.
Thorough
and cutting-edge, Programmed Cell Death: Methods and Protocols is a
comprehensive and valuable resource for researchers, ranging from beginner to
expert, in their studies on programmed cell death.
Keywords
- delineating the molecular mechanisms
- Drosophila
- metazoan apoptosis process
- apoptosis detection techniques
- autophagic cell death processes
- caspase activity initiation at single cell level
- peptide-based techniques for detecting caspases
- Bcl-2 family protein dynamics
- protein-protein interactions
- genome editing
- apoptosis
Editors and Affiliations
-
Department of Biochemistry and Genetics, La Trobe Inst for Mol Sci, La Trobe Univ, Bundoora, Australia
Hamsa Puthalakath, Christine J. Hawkins
Bibliographic Information
Book Title: Programmed Cell Death
Book Subtitle: Methods and Protocols
Editors: Hamsa Puthalakath, Christine J. Hawkins
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3581-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3579-6Published: 23 April 2016
Softcover ISBN: 978-1-4939-8084-0Published: 21 April 2018
eBook ISBN: 978-1-4939-3581-9Published: 23 April 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 291
Number of Illustrations: 20 b/w illustrations, 39 illustrations in colour
Topics: Cell Biology