Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1413)
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Table of contents (25 protocols)
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Front Matter
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Methods Focused on Cytology
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Front Matter
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Methods Focused on Microtubules and the Mitotic Spindle
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Front Matter
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Methods Focused on Kinetochores and the Kinetochore-Microtubule Interface
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Front Matter
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Methods Focused on the Spindle Pole
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Front Matter
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Methods Focused on the Cellular Functions of Microtubule Motor Proteins
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Front Matter
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About this book
This volume includes a series of
protocols focused on mitotic spindle assembly and function. The methods covered
in this book feature a broad range of techniques from basic microscopy to the
study of spindle physiologies relevant to cancer. These methods can be applied
to diverse model systems that range from the cell-free Xenopus egg
extract system to the moss Physcomitrella patens, in an effort to
demonstrate the key contributions made by researchers using multiple model
organisms. Chapters in The Mitotic Spindle: Methods and Protocols integrate cutting-edge technologies that have only become available due to the
cross-disciplinary efforts, such as ATP analogue sensitive inhibition of
mitotic kinases. Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips ontroubleshooting and avoiding
known pitfalls.
Thorough and informative, The
Mitotic Spindle: Methods and Protocols, is a valuable resource for
researchers who are new to mitosis or are already experts in the field.
Editors and Affiliations
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Massachusetts Institute of Technolo, Cambridge, USA
Paul Chang
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Dept of Cell & Developmental Biolog, Vanderbilt University, Nashville, USA
Ryoma Ohi
Bibliographic Information
Book Title: The Mitotic Spindle
Book Subtitle: Methods and Protocols
Editors: Paul Chang, Ryoma Ohi
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3542-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3540-6Published: 20 May 2016
Softcover ISBN: 978-1-4939-8072-7Published: 26 May 2018
eBook ISBN: 978-1-4939-3542-0Published: 19 May 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 424
Number of Illustrations: 27 b/w illustrations, 52 illustrations in colour
Topics: Membrane Biology, Biological Microscopy