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Endocannabinoid Signaling

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Mauro Maccarrone

    1. Campus Bio-Medico University of Rome, Department of Medicine, Rome, Italy

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implelementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1412)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Need for Methods to Investigate Endocannabinoid Signaling

    • Mauro Maccarrone
    Pages 1-8

  3. Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues

    • Laura Bindila, Beat Lutz
    Pages 9-18

  4. Determination of 2-Arachidonoylglycerol by μSPE-LC-MS/MS

    • Natalia Battista, Manuel Sergi
    Pages 19-26

  5. Analysis of Omega-3 Fatty Acid Derived N-Acylethanolamines in Biological Matrices

    • Renger F. Witkamp, Michiel G. J. Balvers
    Pages 27-40

  6. Assay of CB1 Receptor Binding

    • Valeria M. Catani, Valeria Gasperi
    Pages 41-55

  7. The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells

    • Maria Grazia Cascio, Pietro Marini, Roger G. Pertwee
    Pages 57-63

  8. Assay of TRPV1 Receptor Signaling

    • Aniello Schiano Moriello, Luciano De Petrocellis
    Pages 65-76

  9. A Functional Assay for GPR55: Envision Protocol

    • Sharon Anavi-Goffer, Ruth A. Ross
    Pages 77-83

  10. The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells

    • Pietro Marini, Maria Grazia Cascio, Roger G. Pertwee
    Pages 85-93

  11. Assay of GTPγS Binding in Autoradiography

    • Marina Gabaglio, Pamela Prini, Erica Zamberletti, Tiziana Rubino, Daniela Parolaro
    Pages 95-101

  12. Protocol to Study β-Arrestin Recruitment by CB1 and CB2 Cannabinoid Receptors

    • Marjolein Soethoudt, Noortje van Gils, Mario van der Stelt, Laura H. Heitman
    Pages 103-111

  13. Assay of NAT Activity

    • Toru Uyama, Natsuo Ueda
    Pages 113-122

  14. Assay of NAPE-PLD Activity

    • Filomena Fezza, Nicolina Mastrangelo, Mauro Maccarrone
    Pages 123-130

  15. Assay of FAAH Activity

    • Monica Bari, Monica Feole, Mauro Maccarrone
    Pages 131-136

  16. Assay of NAAA Activity

    • Kazuhito Tsuboi, Natsuo Ueda
    Pages 137-148

  17. Assay of DAGLα/β Activity

    • Tiziana Bisogno
    Pages 149-156

  18. Assay of Monoacylglycerol Lipase Activity

    • Kwang-Mook Jung, Daniele Piomelli
    Pages 157-168

  19. A Sensitive and Versatile Fluorescent Activity Assay for ABHD6

    • Juha R. Savinainen, Dina Navia-Paldanius, Jarmo T. Laitinen
    Pages 169-178

  20. A Sensitive and Versatile Fluorescent Activity Assay for ABHD12

    • Juha R. Savinainen, Dina Navia-Paldanius, Jarmo T. Laitinen
    Pages 179-189
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Keywords

About this book

This volume encompasses all major methodologies to interrogate endocannabinoid systems (ECS) and endocannabinoids (eCBs) signaling. With increasing interest towards the manifold activities of eCBs, this book discusses the chemical, biochemical, and molecular biological assays, and activity of distinct elements of the ECS. These include membrane, nuclear receptors, biosynthetic and hydrolytic enzymes, and membrane transporters and oxidative enzymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Timely and cutting edge, Endocannabinoid Signaling: Methods and Protocols is a valuable resource and will help chemists, drug designers, biochemists, molecular biologists, cell biologists, pharmacologists, and (electro) physiologists navigate the mare magnum of endocannabinoid research.

Editors and Affiliations

  • Campus Bio-Medico University of Rome, Department of Medicine, Rome, Italy

    Mauro Maccarrone

Bibliographic Information

  • Book Title: Endocannabinoid Signaling

  • Book Subtitle: Methods and Protocols

  • Editors: Mauro Maccarrone

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3539-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3537-6Published: 01 June 2016

  • Softcover ISBN: 978-1-4939-8071-0Published: 26 May 2018

  • eBook ISBN: 978-1-4939-3539-0Published: 31 May 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 285

  • Number of Illustrations: 49 b/w illustrations, 26 illustrations in colour

  • Topics: Neurosciences

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