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The Nuclear Envelope

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Sue Shackleton

    1. Department of Biochemistry, University of Leicester, Leicester, United Kingdom

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  2. Philippe Collas

    1. Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway

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    You can also search for this editor in PubMed   Google Scholar

  3. Eric C. Schirmer

    1. Wellcome Trust Centre for Cell Biol, University of Edinburgh, Edinburgh, United Kingdom

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1411)

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Table of contents (31 protocols)

  1. Front Matter

    Pages i-xiv
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  2. Nuclear Envelope Isolation

    1. Front Matter

      Pages 1-1
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    2. Isolation, Proteomic Analysis, and Microscopy Confirmation of the Liver Nuclear Envelope Proteome

      • Nadia Korfali, Laurence Florens, Eric C. Schirmer
      Pages 3-44

    3. Exploring the Protein Composition of the Plant Nuclear Envelope

      • Xiao Zhou, Kentaro Tamura, Katja Graumann, Iris Meier
      Pages 45-65

    4. High-Efficiency Isolation of Nuclear Envelope Protein Complexes from Trypanosomes

      • Samson O. Obado, Mark C. Field, Brian T. Chait, Michael P. Rout
      Pages 67-80

  3. Nuclear Envelope Protein Interactions, Localization, and Dynamics

    1. Front Matter

      Pages 81-81
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    2. Superresolution Microscopy of the Nuclear Envelope and Associated Proteins

      • Wei Xie, Henning F. Horn, Graham D. Wright
      Pages 83-97

    3. Analyses of the Dynamic Properties of Nuclear Lamins by Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS)

      • Shimi Takeshi, Chan-Gi Pack, Robert D. Goldman
      Pages 99-111

    4. Probing Protein Distribution Along the Nuclear Envelope In Vivo by Using Single-Point FRAP

      • Krishna C. Mudumbi, Weidong Yang
      Pages 113-122

    5. The Use of Two-Photon FRET–FLIM to Study Protein Interactions During Nuclear Envelope Fusion In Vivo and In Vitro

      • Richard D. Byrne, Banafshé Larijani, Dominic L. Poccia
      Pages 123-132

    6. Identifying Protein-Protein Associations at the Nuclear Envelope with BioID

      • Dae In Kim, Samuel C. Jensen, Kyle J. Roux
      Pages 133-146

    7. In Situ Detection of Interactions Between Nuclear Envelope Proteins and Partners

      • Alice Barateau, Brigitte Buendia
      Pages 147-158

    8. Methods for Single-Cell Pulse-Chase Analysis of Nuclear Components

      • Marek Drozdz, David J. Vaux
      Pages 159-176

    9. Analysis of Nuclear Lamina Proteins in Myoblast Differentiation by Functional Complementation

      • Olga Tapia, Larry Gerace
      Pages 177-194

    10. Analysis of Meiotic Telomere Behavior in the Mouse

      • Jana Link, Ricardo Benavente, Manfred Alsheimer
      Pages 195-208

  4. Nuclear Envelope Interactions with the Cytoskeleton

    1. Front Matter

      Pages 209-209
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    2. Identification and Validation of Putative Nesprin Variants

      • Flavia Autore, Catherine M. Shanahan, Qiuping Zhang
      Pages 211-220

    3. Detection of Diverse and High Molecular Weight Nesprin-1 and Nesprin-2 Isoforms Using Western Blotting

      • James Carthew, Iakowos Karakesisoglou
      Pages 221-232

    4. The Use of Polyacrylamide Hydrogels to Study the Effects of Matrix Stiffness on Nuclear Envelope Properties

      • Rose-Marie Minaisah, Susan Cox, Derek T. Warren
      Pages 233-239

    5. Cell Microharpooning to Study Nucleo-Cytoskeletal Coupling

      • Gregory Fedorchak, Jan Lammerding
      Pages 241-254
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Keywords

About this book

This volume provides a wide range of protocols used in studying the nuclear envelope, with special attention to the experimental adjustments that may be required to successfully investigate this complex organelle in cells from various organisms. The Nuclear Envelope: Methods and Protocols is divided into five sections: Part I – Nuclear Envelope Isolation; Part II – Nuclear Envelope Protein Interactions, Localization, and Dynamics; Part III – Nuclear Envelope Interactions with the Cytoskeleton; Part IV – Nuclear Envelope-Chromatin Interactions; and Part V – Nucleo-Cytoplasmic Transport. Many of the modifications discussed in this book have only been circulated within laboratories that have conducted research in this field for many years. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, The Nuclear Envelope: Methods and Protocols is a timely resource for researchers who have joined this dynamic and rapidly growing field. 

Editors and Affiliations

  • Department of Biochemistry, University of Leicester, Leicester, United Kingdom

    Sue Shackleton

  • Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway

    Philippe Collas

  • Wellcome Trust Centre for Cell Biol, University of Edinburgh, Edinburgh, United Kingdom

    Eric C. Schirmer

Bibliographic Information

  • Book Title: The Nuclear Envelope

  • Book Subtitle: Methods and Protocols

  • Editors: Sue Shackleton, Philippe Collas, Eric C. Schirmer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3530-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3528-4Published: 05 May 2016

  • Softcover ISBN: 978-1-4939-8068-0Published: 26 May 2018

  • eBook ISBN: 978-1-4939-3530-7Published: 04 May 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 523

  • Number of Illustrations: 26 b/w illustrations, 77 illustrations in colour

  • Topics: Cell Biology

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