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  • © 2016

Optogenetics

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1408)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xiii
  2. Natural Resources for Optogenetic Tools

    • Tilo Mathes
    Pages 19-36
  3. Controlling Protein Activity and Degradation Using Blue Light

    • Anne P. Lutz, Christian Renicke, Christof Taxis
    Pages 67-78
  4. Photo Control of Protein Function Using Photoactive Yellow Protein

    • Jakeb M. Reis, G. Andrew Woolley
    Pages 79-92
  5. A Fluorometric Activity Assay for Light-Regulated Cyclic-Nucleotide-Monophosphate Actuators

    • Charlotte Helene Schumacher, Heinz G. Körschen, Christopher Nicol, Carlos Gasser, Reinhard Seifert, Martin Schwärzel et al.
    Pages 93-105
  6. Optogenetic Control of Pancreatic Islets

    • Thomas M. Reinbothe, Inês G. Mollet
    Pages 107-123
  7. Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression

    • Rocio Ochoa-Fernandez, Sophia L. Samodelov, Simon M. Brandl, Elke Wehinger, Konrad Müller, Wilfried Weber et al.
    Pages 125-139
  8. Enhancing Channelrhodopsins: An Overview

    • Jonas Wietek, Matthias Prigge
    Pages 141-165
  9. Optogenetics in Drosophila Neuroscience

    • Thomas Riemensperger, Robert J. Kittel, André Fiala
    Pages 167-175
  10. Optogenetic Control of Mammalian Ion Channels with Chemical Photoswitches

    • Damien Lemoine, Romain Durand-de Cuttoli, Alexandre Mourot
    Pages 177-193
  11. Optogenetic Modulation of Locomotor Activity on Free-Behaving Rats

    • Kedi Xu, Jiacheng Zhang, Songchao Guo, Xiaoxiang Zheng
    Pages 195-206
  12. Combined Optogenetic and Chemogenetic Control of Neurons

    • Ken Berglund, Jack K. Tung, Bryan Higashikubo, Robert E. Gross, Christopher I. Moore, Ute Hochgeschwender
    Pages 207-225
  13. Optogenetic Approaches for Mesoscopic Brain Mapping

    • Michael Kyweriga, Majid H. Mohajerani
    Pages 251-265
  14. Optogenetic Tools for Confined Stimulation in Deep Brain Structures

    • Alexandre Castonguay, Sébastien Thomas, Frédéric Lesage, Christian Casanova
    Pages 267-279

About this book

This volume provides researchers with up-to-date protocols and takes a close look at current research and promising applications. Optogenetics: Methods and Protocols contains a collection of recently developed technical protocols on optogenetic applications in neuroscience, brain mapping, treatment of neurological disorders, and restoration of visual function. Several introductory and discussion chapters offer a wide overview about sources and diversity of optogenetic tools, design strategies, and potential applications in other fields like plant research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, Optogenetic: Methods and Protocols, delivers an interdisciplinary view of optogenetic applications and its potential to develop as a valuable tool for basic research, as well as biomedical and biotechnological applications.

 

Editors and Affiliations

  • University of Bielefeld, Dept of Cellular & Dev Biology of Plants, Bielefeld, Germany

    Arash Kianianmomeni

Bibliographic Information

  • Book Title: Optogenetics

  • Book Subtitle: Methods and Protocols

  • Editors: Arash Kianianmomeni

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3512-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3510-9Published: 11 March 2016

  • Softcover ISBN: 978-1-4939-8062-8Published: 07 June 2018

  • eBook ISBN: 978-1-4939-3512-3Published: 10 March 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 408

  • Number of Illustrations: 13 b/w illustrations, 98 illustrations in colour

  • Topics: Neurosciences, Human Genetics

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access