Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1407)
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Table of contents (27 protocols)
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Front Matter
About this book
This second edition expands on the first edition with new chapters describing methods for studying
cell movement, molecular components involved in chemotaxis, spatiotemporal
dynamics of signaling components, and quantitative modeling, as well as several
updated chapters from the first edition. Various methods to investigate
directional cell growth and movements are presented in Chapters 1-20. These
chapters contains experimental procedures to visualize and measure migration
behaviors of different kinds of organisms, including chemotropism in the
budding yeast; cell growth and migration of D. discoideum; border cell
migration in Drosophila; chemotaxis of mouse and human neutrophils; and
HIV-induced T cell chemotactic response. Chemotaxis: Methods and Protocols,
Second Edition also contains microscopy procedures for studying breast cancer
cell migration, tumor cell invasion in vivo, and axon guidance. The book concludes
with Chapters 21-27 describing methods that measure spatiotemporal dynamics of
signaling components involved in chemotaxis; introduce imaging techniques, such
as TRIF, BRET, FRET, and single-molecule microscopy; and mathematical models of
experimentally generated chemoattractant gradients. Written in the highly
successful Methods in Molecular Biology series format, chapters include
introductions to their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols, and tips on
troubleshooting and avoiding known pitfalls.
Cutting edge and thorough,
Chemotaxis: Methods and Protocols, Second Edition is a valuable resource for
anyone who is interested in the diverse methodologies that are propelling
chemotaxis research forward.
Editors and Affiliations
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Nat Inst of Allergy& Infectious Dis, NIH, Laboratory of Immunogenetics, Rockville, USA
Tian Jin
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National Institutes of Health, Nat Inst Alcohol Abuse & Alcoholism, Rockville, USA
Dale Hereld
Bibliographic Information
Book Title: Chemotaxis
Book Subtitle: Methods and Protocols
Editors: Tian Jin, Dale Hereld
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3480-5
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3478-2Published: 07 June 2016
Softcover ISBN: 978-1-4939-8059-8Published: 26 May 2018
eBook ISBN: 978-1-4939-3480-5Published: 06 June 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XIV, 425
Number of Illustrations: 35 b/w illustrations, 83 illustrations in colour
Topics: Cell Biology