Skip to main content
  • Book
  • © 2016

Breast Cancer

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1406)

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (25 protocols)

  1. Front Matter

    Pages i-xii
  2. Detection of Molecular Markers of Breast Cancer

    1. Front Matter

      Pages 1-1
    2. Clinical Applications for Immunohistochemistry of Breast Lesions

      • Kester Haye, Rajarsi Gupta, Christopher Metter, Jingxuan Liu
      Pages 11-37
    3. Immunohistochemistry for Triple-Negative Breast Cancer

      • Kalnisha Naidoo, Sarah E. Pinder
      Pages 39-51
    4. In Situ Hybridization of Breast Cancer Markers

      • Li Min, Chengchao Shou
      Pages 53-59
  3. Genetic Detection for Breast Cancer

    1. Front Matter

      Pages 71-71
    2. Quantification of mRNA Levels Using Real-Time Polymerase Chain Reaction (PCR)

      • Yiyi Li, Kai Wang, Longhua Chen, Xiaoxia Zhu, Jie Zhou
      Pages 73-79
    3. Pyrosequencing Analysis for Breast Cancer DNA Methylome

      • Cem Kuscu, Canan Kuscu
      Pages 89-104
  4. Isolation of Breast Cancer Cells

    1. Front Matter

      Pages 105-105
    2. Vita-Assayâ„¢ Method of Enrichment and Identification of Circulating Cancer Cells/Circulating Tumor Cells (CTCs)

      • Shaun Tulley, Qiang Zhao, Huan Dong, Michael L. Pearl, Wen-Tien Chen
      Pages 107-119
    3. Breast Cancer Stem Cell Isolation

      • Xuanmao Jiao, Albert A. Rizvanov, Massimo Cristofanilli, Regina R. Miftakhova, Richard G. Pestell
      Pages 121-135
  5. In Vitro Experimental Models for Breast Cancer

    1. Front Matter

      Pages 137-137
    2. Cellular Apoptosis Assay of Breast Cancer

      • Yu Sun, Wei-Xing Zong
      Pages 139-149
    3. Assessment of Synthetic Matrix Metalloproteinase Inhibitors by Fluorogenic Substrate Assay

      • Ty J. Lively, Dale B. Bosco, Zahraa I. Khamis, Qing-Xiang Amy Sang
      Pages 161-170
    4. Determination of Breast Cancer Cell Migratory Ability

      • David Schmitt, Joel Andrews, Ming Tan
      Pages 171-180
    5. A Novel Collagen Dot Assay for Monitoring Cancer Cell Migration

      • Vincent M. Alford, Eric Roth, Qian Zhang, Jian Cao
      Pages 181-187

About this book

This volume provides resources, ideas, and bench manuals for the study of breast cancer. This book is divided into five sections: methods used in clinical laboratory for diagnosis (Detection of Molecular Markers of Breast Cancer); methods used in both clinical and research laboratories for testing genetic alterations (Genetic Detection of Breast Cancer) and (Isolation of Breast Cancer Cells); methods used to study the behavior of breast cancer cells (In Vitro Experimental Assays for Breast Cancer); and methods used for mimicking human breast cancer in a living organism (In Vivo Experimental Models for Breast Cancer). Breast Cancer: Methods and Protocols also explores several recently developed techniques for the study of breast cancer progression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, Breast Cancer: Methods and Protocols, is a valuable handbook for both graduate and advanced undergraduate students of biological sciences, as well as scientists, technicians, and physicians working in the academic, hospital, or pharmaceutical industry aimed at studying or diagnosing breast cancer.

Editors and Affiliations

  • School of Medicine, Stony Brook University, Stony Brook, USA

    Jian Cao

About the editor

JIAN CAO Room 004, Life Sciences Building Division of Cancer Prevention Department of Medicine School of Medicine, Stony Brook University Stony Brook, NY 11794-5200 631-632-1815 email: Jian.Cao@stonybrookmedicine.edu

Bibliographic Information

  • Book Title: Breast Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Jian Cao

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3444-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3442-3Published: 29 January 2016

  • Softcover ISBN: 978-1-4939-8052-9Published: 01 April 2018

  • eBook ISBN: 978-1-4939-3444-7Published: 28 January 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 332

  • Number of Illustrations: 13 b/w illustrations, 58 illustrations in colour

  • Topics: Cancer Research

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access