Skip to main content
SpringerLink
Log in

Clinical Applications of PCR

  • Book
  • © 2016

Overview

Editors:
  1. Rajyalakshmi Luthra

    1. Anderson Cancer Center, The University of Texas MD, Houston, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Rajesh R. Singh

    1. Anderson Cancer Center, The University of Texas MD, Houston, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  3. Keyur P. Patel

    1. Anderson Cancer Center, The University of Texas MD, Houston, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes fully updated, cutting-edge methods and protocols for PCR in a clinical setting
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1392)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. A Targeted Q-PCR-Based Method for Point Mutation Testing by Analyzing Circulating DNA for Cancer Management Care

    • Alain R. Thierry
    Pages 1-16

  3. COLD-PCR: Applications and Advantages

    • Zhuang Zuo, Kausar J. Jabbar
    Pages 17-25

  4. PCR-Based Detection of DNA Copy Number Variation

    • Meenakshi Mehrotra
    Pages 27-32

  5. Emulsion PCR: Techniques and Applications

    • Rashmi Kanagal-Shamanna
    Pages 33-42

  6. Digital PCR: Principles and Applications

    • Rashmi Kanagal-Shamanna
    Pages 43-50

  7. Quantitative PCR for Plasma Epstein-Barr Virus Loads in Cancer Diagnostics

    • Sanam Loghavi
    Pages 51-61

  8. High-Resolution Melt Curve Analysis in Cancer Mutation Screen

    • Meenakshi Mehrotra, Keyur P. Patel
    Pages 63-69

  9. Locked Nucleic Acid Probes (LNA) for Enhanced Detection of Low-Level, Clinically Significant Mutations

    • Khedoudja Nafa, Meera Hameed, Marie E. Arcila
    Pages 71-82

  10. Genotyping of Frequent Mutations in Solid Tumors by PCR-Based Single-Base Extension and MassARRAY Analysis

    • Alberto Paniz Mondolfi, Rajesh R. Singh
    Pages 83-101

  11. Microfluidics-Based PCR for Fusion Transcript Detection

    • Hui Chen
    Pages 103-111

  12. Polymerase Chain Reaction Diagnosis of Leishmaniasis: A Species-Specific Approach

    • Eglys González-Marcano, Hirotomo Kato, Juan Luis Concepción, María Elizabeth Márquez, Alberto Paniz Mondolfi
    Pages 113-124

  13. Detection of Trypanosoma cruzi by Polymerase Chain Reaction

    • María Elizabeth Márquez, Juan Luis Concepción, Eglys González-Marcano, Alberto Paniz Mondolfi
    Pages 125-141

  14. PCR Techniques in Next-Generation Sequencing

    • Rashmi S. Goswami
    Pages 143-151

  15. Single-Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics

    • Chi Young Ok, Rajesh R. Singh, Alaa A. Salim
    Pages 153-159

  16. Quantitative Real-Time PCR: Recent Advances

    • Charanjeet Singh, Sinchita Roy-Chowdhuri
    Pages 161-176

  17. PCR Techniques in Characterizing DNA Methylation

    • Khalida Wani, Kenneth D. Aldape
    Pages 177-186

  18. Back Matter

    Pages 187-191
    Download chapter PDF
Back to top

Keywords

About this book

This updated volume explores a wide variety of clinical applications of PCR such as detecting DNA methylation, detection of viruses and protozoa in infectious diseases, estimation of gene copy number aberrations, primer extension coupled with mass spectroscopy, and high throughput NGS techniques. The application of PCR has shown incredible value in the study of genomics and transcriptomics, not only for discovery but also for routine clinical applications, and it forms the cornerstone of personalized medicine. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and authoritative, Clinical Applications of PCR, Third Edition serves as an ideal guide for researchers aiming to understand the principles behind each application and for their implementation in the laboratory.

Reviews

“This multi-author treatise reviews the clinical applications of PCR in various fields of medicine stressing its importance for genetic analysis. It has 16 chapters, each with color illustrations and references. Recommended for researchers in biology and hospital laboratories.” (Pediatric Endocrinology Reviews (PER), Vol. 14 (3), March, 2017)

Editors and Affiliations

  • Anderson Cancer Center, The University of Texas MD, Houston, USA

    Rajyalakshmi Luthra, Rajesh R. Singh, Keyur P. Patel

Bibliographic Information

  • Book Title: Clinical Applications of PCR

  • Editors: Rajyalakshmi Luthra, Rajesh R. Singh, Keyur P. Patel

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3360-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3358-7Published: 03 February 2016

  • Softcover ISBN: 978-1-4939-8030-7Published: 31 March 2018

  • eBook ISBN: 978-1-4939-3360-0Published: 03 February 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: X, 191

  • Number of Illustrations: 9 b/w illustrations, 16 illustrations in colour

  • Topics: Cell Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation