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Kidney Research

Experimental Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Tim D. Hewitson

    1. Royal Hospital Melbourne Dept. Nephrology, Melbourne, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. Edward R. Smith

    1. Department of Nephrology, Royal Melbourne Hospital, Parkville, Melbourne, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  3. Stephen G. Holt

    1. Department of Nephrology, Royal Melbourne Hospital, Parkville, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1397)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
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  2. In Vitro, In Vivo and Ex Vivo Models

    1. Front Matter

      Pages 1-1
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    2. Isolation and Primary Culture of Murine Podocytes with Proven Origin

      • Bart Smeets, Nazanin Kabgani, Marcus J. Moeller
      Pages 3-10

    3. Propagation and Culture of Human Renal Fibroblasts

      • Sven-Jean Tan, Tim D. Hewitson
      Pages 11-23

    4. Isolation and Propagation of Rat Peritoneal Mesothelial Cells

      • Qin Zhou, Xueqing Yu
      Pages 25-34

    5. Rat Models of Acute and/or Chronic Peritoneal Injuries Including Peritoneal Fibrosis and Peritoneal Dialysis Complications

      • Masashi Mizuno, Yasuhiko Ito
      Pages 35-43

    6. Renal Sympathetic Denervation in Rats

      • Masahiro Eriguchi, Kazuhiko Tsuruya
      Pages 45-52

    7. Decellularization of Rat Kidneys to Produce Extracellular Matrix Scaffolds

      • Mei Jin, Yu Yaling, Wang Zhibin, Zhang Jianse
      Pages 53-63

  3. Imaging Techniques

    1. Front Matter

      Pages 65-65
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    2. Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI

      • Kevin M. Bennett, Scott C. Beeman, Edwin J. Baldelomar, Min Zhang, Teresa Wu, Bradley D. Hann et al.
      Pages 67-79

    3. Biopsychronology: A Method Using Live Tissue Staining to Image Cell Function in the Kidney

      • Muhammad Imtiaz Ashraf, Dietmar Fries, Werner Streif, Felix Aigner, Paul Hengster, Jakob Troppmair et al.
      Pages 81-90

  4. In Vivo Analysis of Metabolism in Ischemia-Reperfusion

    1. Front Matter

      Pages 91-91
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    2. Prolonged and Continuous Measurement of Kidney Oxygenation in Conscious Rats

      • Maarten P. Koeners, Connie P. C. Ow, David M. Russell, Roger G. Evans, Simon C. Malpas
      Pages 93-111

    3. Magnetic Resonance Imaging (MRI) Analysis of Ischemia/Reperfusion in Experimental Acute Renal Injury

      • Andreas Pohlmann, Karen Arakelyan, Erdmann Seeliger, Thoralf Niendorf
      Pages 113-127

    4. Assessment of Renal Hemodynamics and Oxygenation by Simultaneous Magnetic Resonance Imaging (MRI) and Quantitative Invasive Physiological Measurements

      • Kathleen Cantow, Karen Arakelyan, Erdmann Seeliger, Thoralf Niendorf, Andreas Pohlmann
      Pages 129-154

    5. Intravital Multiphoton Imaging of the Kidney: Tubular Structure and Metabolism

      • David M. Small, Washington Y. Sanchez, Glenda C. Gobe
      Pages 155-172
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Keywords

About this book

This second edition expands upon the previous volume with additional emphasis on recent innovation in basic renal research and has a more holistic approach on associated disorders such as complications associated with peritoneal dialysis, ischemic acute kidney injury, the sympathetic nervous system, and vascular calcification. The book is divided into five parts: Part I provides a number of in vitro, in vivo, and ex vivo models of kidney disease and associated complications; Part II looks at recent advances in imaging techniques; Part III covers recent developments in studying metabolism in renal ischemia and reperfusion; Part IV addresses study and measurement of vascular calcification; and Part V explores analytical techniques that are both topical and of widespread relevance to the study of experimental renal disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Kidney Research: Experimental Protocols, Second Edition is a valuable collection of protocols useful to new and experienced researchers who are interested in the field of Nephrology.

Editors and Affiliations

  • Royal Hospital Melbourne Dept. Nephrology, Melbourne, Australia

    Tim D. Hewitson

  • Department of Nephrology, Royal Melbourne Hospital, Parkville, Melbourne, Australia

    Edward R. Smith

  • Department of Nephrology, Royal Melbourne Hospital, Parkville, Australia

    Stephen G. Holt

Bibliographic Information

  • Book Title: Kidney Research

  • Book Subtitle: Experimental Protocols

  • Editors: Tim D. Hewitson, Edward R. Smith, Stephen G. Holt

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3353-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3351-8Published: 17 December 2015

  • Softcover ISBN: 978-1-4939-8028-4Published: 29 March 2019

  • eBook ISBN: 978-1-4939-3353-2Published: 16 December 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 292

  • Number of Illustrations: 52 b/w illustrations, 14 illustrations in colour

  • Topics: Molecular Medicine, Nephrology, Pathology

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