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Tumor Immunology

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Attilio Bondanza

    1. Division of Regenerative Medicine, S. Raffaele Scientific Institute, Milano, Italy

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    You can also search for this editor in PubMed   Google Scholar

  2. Monica Casucci

    1. Stem Cells and Gene Therapy, S. Raffaele Scientific Institute Division of Regenerative Medicine, Milano, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1393)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. ATMPs for Cancer Immunotherapy: A Regulatory Overview

    • Maria Cristina Galli
    Pages 1-9

  3. Natural Antibodies to Tumor-Associated Antigens

    • Sheila María Álvarez-Fernández, Lucia De Monte, Massimo Alessio
    Pages 11-25

  4. Generation and Cryopreservation of Clinical Grade Wilms’ Tumor 1 mRNA-Loaded Dendritic Cell Vaccines for Cancer Immunotherapy

    • Evelien L. J. M. Smits, Barbara Stein, Griet Nijs, Eva Lion, Viggo F. Van Tendeloo, Yannick Willemen et al.
    Pages 27-35

  5. Quantitative and Qualitative Analysis of Tumor-Associated CD4+ T Cells

    • Giulia Di Lullo, Lucia De Monte, Claudio Doglioni, Maria Pia Protti
    Pages 37-51

  6. Detection and Functional Analysis of Tumor-Derived LXR Ligands

    • Raffaella Fontana, Aida Paniccia, Vincenzo Russo
    Pages 53-65

  7. In Vitro Generation of Antigen-Specific T Cells from Induced Pluripotent Stem Cells of Antigen-Specific T Cell Origin

    • Shin Kaneko
    Pages 67-73

  8. Chimeric Antigen Receptors for Cancer Immunotherapy

    • Claudia Geldres, Barbara Savoldo, Gianpietro Dotti
    Pages 75-86

  9. Cancer and Chemokines

    • Nicoletta Caronni, Benedetta Savino, Camilla Recordati, Alessandro Villa, Massimo Locati, Raffaella Bonecchi
    Pages 87-96

  10. T Cells as Antigen Carriers for Anti-tumor Vaccination

    • Catia Traversari, Vincenzo Russo
    Pages 97-104

  11. Exploiting Secreted Luciferases to Monitor Tumor Progression In Vivo

    • Laura Falcone, Monica Casucci
    Pages 105-111

  12. Efficient RNA Interference of Primary Leukemic Cells for Loss-of-Function Studies in Xenograft Mouse Models

    • B. Nicolis di Robilant, Maddalena Noviello
    Pages 113-126

  13. Modeling Human Graft-Versus-Host Disease in Immunocompromised Mice

    • Margherita Norelli, Barbara Camisa, Attilio Bondanza
    Pages 127-132

  14. ICOS Expression as Immunologic Marker in Immune Activating Monoclonal Antibodies

    • Riccardo Danielli, Ornella Cutaia, Carolina Fazio, Erika Bertocci, Ester Fonsatti, Michele Maio et al.
    Pages 133-139

  15. Identifying NK Alloreactive Donors for Haploidentical Hematopoietic Stem Cell Transplantation

    • Loredana Ruggeri, Antonella Mancusi, Elena Urbani, Andrea Velardi
    Pages 141-145

  16. Human Microtumors Generated in 3D: Novel Tools for Integrated In Situ Studies of Cancer Immunotherapies

    • Lothar Hambach, Andreas Buser, Marcel Vermeij, Nadine Pouw, Theo van der Kwast, Els Goulmy
    Pages 147-161

  17. Erratum to: Efficient RNA Interference of Primary Leukemic Cells for Loss-of-Function Studies in Xenograft Mouse Models

    • B. Nicolis di Robilant, Maddalena Noviello
    Pages E1-E1

  18. Back Matter

    Pages 163-165
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Keywords

About this book

This volume explores the still undiscovered secrets of tumor immunology and cancer immunology by discussing the methods and techniques that world-renowned experts in the field use in their laboratories.  This book provides a better understanding of the rules governing tumor and cancer immunology, and discusses innovations in the technology of the immunological “smart bullets” (monoclonal antibodies, vaccines, tumor-reactive T cells) used to specifically target cancer cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Tumor Immunology: Methods and Protocols contains a wide breadth of subject coverage that any scientist, clinician, or industry professional interested in this field will find valuable.

Editors and Affiliations

  • Division of Regenerative Medicine, S. Raffaele Scientific Institute, Milano, Italy

    Attilio Bondanza

  • Stem Cells and Gene Therapy, S. Raffaele Scientific Institute Division of Regenerative Medicine, Milano, Italy

    Monica Casucci

Bibliographic Information

  • Book Title: Tumor Immunology

  • Book Subtitle: Methods and Protocols

  • Editors: Attilio Bondanza, Monica Casucci

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3338-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2016

  • Hardcover ISBN: 978-1-4939-3336-5Published: 02 April 2016

  • Softcover ISBN: 978-1-4939-8024-6Published: 14 April 2018

  • eBook ISBN: 978-1-4939-3338-9Published: 31 March 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 165

  • Number of Illustrations: 6 b/w illustrations, 17 illustrations in colour

  • Topics: Cancer Research

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