Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1390)
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Table of contents (25 protocols)
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Front Matter
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Toll-Like Receptor Detection and Activation
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Front Matter
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Toll-Like Receptor Cross-Priming of Associated Receptors
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Front Matter
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Toll-Like Receptor Post-Transcriptional Regulation
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Front Matter
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Toll-Like Receptors and System Control
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Front Matter
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About this book
This second edition volume expands on the first edition with new developments on Toll-Like Receptors (TLRs) controlling events such as cross-priming of associated pattern recognition receptors, post-transcriptional regulation, interaction with other cellular and biologic systems, and cancer progression. This book is divided into five sections: Part I outlines methods for TLR detection, interaction, and intracellular trafficking; Part II describes methods and assays to investigate how TLRs cross-prime other pattern recognition receptors, including intracellular DNA receptors and inflammasome formation; Part III highlights RNA regulation, detailing how TLRs can induce RNA transcripts and molecules such as lncRNAs and microRNAs; Part IV explores TLR detection and activation in systems such as epithelial barrier function, metabolism and the circadian clock, as well as cellular systems including T and B lymphocytes; and Part V describes models to delineate the role of TLRs in diseases such as dermatitis, arthritis, and gastric cancer. Written in the highly successful Methods in Molecular Biology series format, each chapter contains a summary, a list of required materials, step-by-step, readily reproducible laboratory protocols, useful notes to investigate TLRs in cell culture, systems and disease, and tips on troubleshooting and avoiding known pitfalls.
Practical and cutting-edge, Toll-Like Receptors: Methods and Protocols, Second Edition is a valuable resource to any immunologist, molecular or medical biologist working in a laboratory setting. It will add skill to both students and the more advanced molecular biologist who wishes to learn a new technique or move to a different area within their current repertoire of practical knowledge.Editors and Affiliations
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Hudson Institute of Medical Research, Monash University Faculty of Medicine, Clayton, Australia
Claire E. McCoy
Bibliographic Information
Book Title: Toll-Like Receptors
Book Subtitle: Practice and Methods
Editors: Claire E. McCoy
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3335-8
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3333-4Published: 24 January 2016
Softcover ISBN: 978-1-4939-8023-9Published: 31 March 2018
eBook ISBN: 978-1-4939-3335-8Published: 23 January 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XIV, 455
Number of Illustrations: 29 b/w illustrations, 29 illustrations in colour
Topics: Immunology, Receptors