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Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition

  • Book
  • © 2016

Overview

Editors:
  1. S.Mohan Jain

    1. Applied Biology, Univ of Helsinki, Helsinki, Finland

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1391)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Protocols for In Vitro Mass Multiplication and Analysis of Medicinally Important Phenolics of a Salep Orchid, Satyrium nepalense D.Don (“Salam Mishri”)

    • Shashi B. Babbar, Deepak K. Singh
    Pages 1-11

  3. In Vitro Culture and Phytochemical Analysis of Passiflora tenuifila Killip and Passiflora setacea DC (Passifloraceae)

    • Jenny Sumara Sozo, Daniel Cuzziol Cruz, Ana Flavia Pavei, Isadora Medeiros da Costa Pereira, Marcia Wolfart, Fernanda Ramlov et al.
    Pages 13-30

  4. Shoot Tip Meristem Cryopreservation of Hypericum Species

    • Katarína Bruňáková, Eva Čellárová
    Pages 31-46

  5. Protocols for Biotechnological Interventions in Improvement of Vanilla (Vanilla planifolia Andrews.)

    • Minoo Divakaran, K. Nirmal Babu, K. V. Peter
    Pages 47-63

  6. Assessment of In Vitro Biological Activities of Anthocyanins-Rich Plant Species Based on Plinia cauliflora Study Model

    • Heloisa S. Pitz, Adriana C. D. Trevisan, Fausto R. Cardoso, Aline Pereira, Eduardo L. G. Moreira, Manuel A. de Prá et al.
    Pages 65-80

  7. Biotechnological Approaches for Biomass and Cardenolide Production in Digitalis purpurea L.

    • Naivy Pérez-Alonso, Borys Chong-Pérez, Alina Capote, Anabel Pérez, André Gerth, Geert Angenon et al.
    Pages 81-102

  8. In Vitro Regeneration of Endangered Medicinal Plant Heliotropium kotschyi (Ramram)

    • Manal Ahmed Sadeq, Malabika Roy Pathak, Ahmed Ali Salih, Mohammed Abido, Asma Abahussain
    Pages 103-112

  9. Micropropagation and Biomass Production of True-to-Type Stevia rebaudiana Bertoni

    • Arpan R. Modi, Vikas Sharma, Ghanshyam Patil, Amritpal S. Singh, N. Subhash, Nitish Kumar
    Pages 113-123

  10. Panax ginseng Adventitious Root Suspension Culture: Protocol for Biomass Production and Analysis of Ginsenosides by High Pressure Liquid Chromatography

    • Hosakatte Niranjana Murthy, Kee Yoeup Paek
    Pages 125-139

  11. Immobilization of Rubia tinctorum L. Suspension Cultures and Biomass Production

    • Pınar Nartop
    Pages 141-151

  12. In Vitro Propagation and Conservation of Bacopa monnieri L.

    • Neelam Sharma, Rakesh Singh, Ruchira Pandey
    Pages 153-171

  13. Establishment, Culture, and Scale-up of Brugmansia candida Hairy Roots for the Production of Tropane Alkaloids

    • Alejandra Beatriz Cardillo, Julián Rodriguez Talou, Ana María Giulietti
    Pages 173-186

  14. Micropropagation, Acclimatization, and Greenhouse Culture of Veratrum californicum

    • Sarah A. White, Jeffrey Adelberg, Jacqueline Naylor-Adelberg, David A. Mann, Ju Yeon Song, Youping Sun
    Pages 187-199

  15. In Vitro Propagation of Withania somnifera (L.) Dunal

    • Pritika Singh, Rupam Guleri, Pratap Kumar Pati
    Pages 201-213

  16. In Vitro Propagation of Sambong (Blumea balsamifera Linn.)

    • Thelma L. Soriano, Evangelina C. Cangao
    Pages 215-227

  17. Production of Gymnemic Acid from Cell Suspension Cultures of Gymnema sylvestre

    • Praveen Nagella, Vijayalaxmi S. Dandin, Hosakatte Niranjana Murthy
    Pages 229-239

  18. Scale-Up of Agrobacterium rhizogenes-Mediated Hairy Root Cultures of Rauwolfia serpentina: A Persuasive Approach for Stable Reserpine Production

    • Shakti Mehrotra, Vikas Srivastava, Manoj K. Goel, Arun K. Kukreja
    Pages 241-257

  19. In Vitro Shoot Cultures and Analysis of Steroidal Lactones in Withania coagulans (Stocks) Dunal

    • Rohit Jain, Sumita Kachhwaha, S. L. Kothari
    Pages 259-273

  20. In Vitro Propagation of Cannabis sativa L. and Evaluation of Regenerated Plants for Genetic Fidelity and Cannabinoids Content for Quality Assurance

    • Hemant Lata, Suman Chandra, Ikhlas A. Khan, Mahmoud A. ElSohly
    Pages 275-288
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Keywords

About this book

This volume provides a detailed step-by-step description of protocols for the establishment of in vitro cultures of important medicinal plants, their mass multiplication in controlled environment, and step-wise secondary metabolite analysis. Many of these protocols also provide a basis for in vitro germplasm conservation or cryopreservation of medicinal plant species. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Applied Biology, Univ of Helsinki, Helsinki, Finland

    S.Mohan Jain

Bibliographic Information

  • Book Title: Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition

  • Editors: S.Mohan Jain

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3332-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2016

  • Hardcover ISBN: 978-1-4939-3330-3Published: 24 April 2016

  • Softcover ISBN: 978-1-4939-8022-2Published: 21 April 2018

  • eBook ISBN: 978-1-4939-3332-7Published: 23 April 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 463

  • Number of Illustrations: 26 b/w illustrations, 126 illustrations in colour

  • Topics: Plant Sciences, Plant Biochemistry

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