Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1388)
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Table of contents (17 protocols)
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Front Matter
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Introductory Chapters
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Front Matter
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Methods to Detect PTEN in Disease
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Front Matter
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Methods to Study PTEN Regulation
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Front Matter
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Methods to Study PTEN Localization
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Front Matter
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Methods to Study PTEN Structure
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Front Matter
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About this book
This volume provides detailed descriptions of prevailing and novel techniques used by experts in the study of PTEN function in disease and biology. The book begins with chapters exploring methods to detect expression levels of PTEN in normal and diseased human specimens; methods to evaluate specific PTEN function in brain cancer; methods that utilize a new biosensor to measure PTEN regulation; and techniques to measure post-transcriptional regulation of PTEN by micoRNAs and ceRNAs. Other chapters present methods describing novel techniques to detect PTEN localization and previously unstudied structural features of PTEN measured through X-Ray Crystallography and Hydrogen Deuterium Exchange Mass Spectrometry. The book concludes with methods to study PTEN function in model organisms including mice and C. elegans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, PTEN: Methods and Protocols is a valuable collection of methodologies and protocols useful to researchers who are interested in the PTEN field.
Editors and Affiliations
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University of Toronto, Princess Margaret Cancer Centre, Toronto, Canada
Leonardo Salmena, Vuk Stambolic
About the editors
Bibliographic Information
Book Title: PTEN
Book Subtitle: Methods and Protocols
Editors: Leonardo Salmena, Vuk Stambolic
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3299-3
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3297-9Published: 11 December 2015
Softcover ISBN: 978-1-4939-8015-4Published: 27 March 2019
eBook ISBN: 978-1-4939-3299-3Published: 11 December 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 324
Number of Illustrations: 28 b/w illustrations, 17 illustrations in colour
Topics: Cancer Research, Human Genetics