Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1385)
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Table of contents (20 protocols)
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Front Matter
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Vectors and Transformation
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Front Matter
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Product Improvements
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Front Matter
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Extraction and Scale-Up
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Front Matter
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Analysis
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Front Matter
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About this book
This volume provides up-to-date scientific achievements from the world’s top researchers. Recombinant Proteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Recombinant Proteins from Plants: Methods and Protocols, Second Edition is an ideal reference for those who are interested in plant molecular biology and molecular farming.
Editors and Affiliations
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Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, London, Canada
Jacqueline MacDonald
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Department of Biology, Western University, London, Canada
Igor Kolotilin
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London, Canada
Rima Menassa
Bibliographic Information
Book Title: Recombinant Proteins from Plants
Book Subtitle: Methods and Protocols
Editors: Jacqueline MacDonald, Igor Kolotilin, Rima Menassa
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3289-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3288-7Published: 27 November 2015
Softcover ISBN: 978-1-4939-5004-1Published: 23 August 2016
eBook ISBN: 978-1-4939-3289-4Published: 27 November 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XI, 296
Number of Illustrations: 31 b/w illustrations, 33 illustrations in colour
Topics: Plant Sciences, Plant Biochemistry